The recombination regulator RecX negatively regulates heat-stable antifungal factor (HSAF) biosynthesis in Lysobacter enzymogenes

Phytopathology Research

Research

Cheng Li¹  Chunlan Xiong¹  Xue Zhou¹  Yong Wang²  Kaihuai Li²  Rongxian Hou³  Fengquan Liu³ 

[1] Department of Plant Pathology, College of Agriculture, Guizhou University, 550025, Guiyang, China;Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, 550025, Guiyang, China;Department of Plant Pathology, College of Agriculture, Guizhou University, 550025, Guiyang, China;Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, 550025, Guiyang, China;Vegetable Research Institute, Guizhou University, 550025, Guiyang, China;Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, 210014, Nanjing, China;

关键词: Heat-stable antifungal factor (HSAF);    Biosynthesis regulator;    RecX;    lafB;    Transcription;    Lysobacter enzymogenes;   

DOI  :  10.1186/s42483-023-00160-6

 received in 2022-11-16, accepted in 2023-01-13,  发布年份 2023

来源: Springer

【 摘 要 】

Bacteria often use multiple transcription factors to regulate specific biological processes. Biosynthesis of heat-stable antifungal factor (HSAF) is regulated by multiple factors in Lysobacter enzymogenes. However, the mechanism of HSAF biosynthesis regulation remains largely unknown. In this study, we screened a potential HSAF biosynthesis regulator, RecX, by a DNA pull-down assay. Deletion of recX resulted in a significant increase in the production of HSAF, and overexpression of recX significantly suppressed HSAF production. Importantly, our results showe that RecX directly binds to the promoter region of the lafB gene to inhibit its transcription and thus decreases HSAF production in L. enzymogenes. These findings reveal the novel mechanism of RecX regulation of antifungal antibiotic production in L. enzymogenes.

【 授权许可】

CC BY   
© The Author(s) 2023

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