Low expression of ZSCAN4 predicts unfavorable outcome in urothelial carcinoma of upper urinary tract and urinary bladder

World Journal of Surgical Oncology

Research

Tzu-Ju Chen¹  Hong-Yue Lai²  Chung-Hsi Hsing³  Ti-Chun Chan⁴  Chien-Feng Li⁵  Wan-Shan Li⁶  Hong-Lin He⁷  Yu-Hsuan Kuo⁸  Kun-Lin Hsieh⁹  Steven K. Huang^1,10  Yow-Ling Shiue^1,11 

[1] Department of Clinical Pathology, Chi Mei Medical Center, Tainan, Taiwan;Department of Medical Technology, Chung Hwa University of Medical Technology, Tainan, Taiwan;Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan;Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan;Department of Anesthesiology, Chi Mei Medical Center, Tainan, Taiwan;Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan;National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan;Department of Medical Research, Chi Mei Medical Center, Tainan, Taiwan;National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan;Institute of Precision Medicine, National Sun Yat-Sen University, Kaohsiung, Taiwan;Department of Medical Technology, Chung Hwa University of Medical Technology, Tainan, Taiwan;Department of Pathology, Chi Mei Medical Center, Tainan, Taiwan;Department of Pathology, E-DA Cancer Hospital, I-Shou University, Kaohsiung, Taiwan;Division of Hematology and Oncology, Department of Internal Medicine, Chi-Mei Medical Center, Tainan, Taiwan;College of Pharmacy and Science, Chia Nan University, Tainan, Taiwan;Division of Urology, Department of Surgery, Chi Mei Medical Center, Tainan, Taiwan;Division of Urology, Department of Surgery, Chi Mei Medical Center, Tainan, Taiwan;Department of Biotechnology, Chia Nan University of Pharmacy and Science, Tainan, Taiwan;Institute of Precision Medicine, National Sun Yat-Sen University, Kaohsiung, Taiwan;

关键词: ZSCAN4;    Urothelial carcinoma;    UC;    Prognosis;    Tumor suppressor;   

DOI  :  10.1186/s12957-023-02948-4

 received in 2022-12-09, accepted in 2023-02-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundWith the advance in genome-wide analyses, genetic alternations have been found to play an important role in carcinogenesis and aggressiveness of UC. Through bioinformatic analysis of gene expression profiles of urinary bladder urothelial carcinoma (UBUC) from publicly available GEO dataset (GSE31684), Zinc finger and SCAN domain containing 4 (ZSCAN4) was identified as a significant downregulated gene in muscle-invasive bladder cancer when compared with non-muscle-invasive bladder cancer.MethodsThe expression of ZSCAN4 was evaluated by immunohistochemistry in 340 upper urinary tract urothelial carcinomas (UTUCs) and 295 UBUCs. The expression profiles of ZSCAN4 and potential signaling pathways were analyzed bioinformatically.ResultsIn UTUC, low expression of ZSCAN4 was significantly associated with advanced primary pT stage (P = 0.011), increased nodal metastasis (P = 0.002) and increased vascular invasion (P = 0.019). In UBUC, low expression of ZSCAN4 was significantly correlated with advanced primary pT stage (P < 0.001), increased nodal metastasis (P = 0.001), high histological grade (P = 0.003) and increased vascular invasion (P = 0.003). In survival analysis, low expression of ZSCAN4 acted as an independent negative prognostic factor for disease-specific survival and metastasis-free survival both in UTUC and UBUC. Gene ontology analysis showed that ZSCAN4 mRNA and its co-downregulated genes are associated with the mitotic cell cycle.ConclusionsLow expression of ZSCAN4 predicted worse outcome in urothelial carcinoma and might have potential regulatory role in cell mitosis.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 4

13690_2023_1046_Article_IEq7.gif

Fig. 1

Fig. 2