Single nucleotide polymorphism (SNP) markers for genetic diversity and population structure study in Ethiopian barley (Hordeum vulgare L.) germplasm

BMC Genomic Data

Research

Aemiro Bezabih Woldeyohannes¹  Mihret Yirgu²  Mulugeta Kebede³  Berhane Lakew⁴  Tileye Feyissa⁵  Mulusew Fikere⁶ 

[1] Debre Birhan Agricultural Research Center, Amhara Agricultural Research Institute, Debre Birhan, Ethiopia;Department of Applied Biology, School of Applied Natural Science, Adama Science and Technology University, P.O.Box 1888, Adama, Ethiopia;Department of Plant Science, College of Agriculture and Natural Resource, Madda Walabu University, P.O.Box 247, Robe, Oromia, Ethiopia;Department of Plant Biology and Biodiversity Management, College of Natural and Computational Sciences, Addis Ababa University, P.O.Box 3434, Addis Ababa, Ethiopia;Holetta Agricultural Research Center, Ethiopian Institute of Agricultural Research, Holetta, Ethiopia;Institute of Biotechnology, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopia;Institute of Molecular Bioscience, University of Queensland, Brisbane, Australia;

关键词: Accession;    Cluster;    Diversity;    Ex-situ;    Genetic differentiation;   

DOI  :  10.1186/s12863-023-01109-6

 received in 2022-08-28, accepted in 2023-01-31,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHigh-density single nucleotide polymorphisms (SNPs) are the most abundant and robust form of genetic variants and hence make highly favorable markers to determine the genetic diversity and relationship, enhancing the selection of breeding materials and the discovery of novel genes associated with economically important traits. In this study, a total of 105 barley genotypes were sampled from various agro-ecologies of Ethiopia and genotyped using 10 K single nucleotide polymorphism (SNP) markers. The refined dataset was used to assess genetic diversity and population structure.ResultsThe average gene diversity was 0.253, polymorphism information content (PIC) of 0.216, and minor allelic frequency (MAF) of 0.118 this revealed a high genetic variation in barley genotypes. The genetic differentiation also showed the existence of variations, ranging from 0.019 to 0.117, indicating moderate genetic differentiation between barley populations. Analysis of molecular variance (AMOVA) revealed that 46.43% and 52.85% of the total genetic variation occurred within the accessions and populations, respectively. The heat map, principal components and population structure analysis further confirm the presence of four distinct clusters.ConclusionsThis study confirmed that there is substantial genetic variation among the different barley genotypes. This information is useful in genomics, genetics and barley breeding.

【 授权许可】

CC BY   
© The Author(s) 2023

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