Lipid biomarkers that reflect postoperative recurrence risk in lung cancer patients who smoke: a case–control study

Lipids in Health and Disease

Research

Takuya Kitamoto¹  Minako Kondo¹  Tomohito Sato²  Tomoaki Kahyo³  Yutaka Takahashi³  Mitsutoshi Setou⁴  Haruhiko Sugimura⁵  Norihiko Shiiya⁶  Keigo Sekihara⁶  Kazuhito Funai⁶  Takamitsu Hayakawa⁶  Yusuke Takanashi⁶  Akikazu Kawase⁶ 

[1] Advanced Research Facilities & Services, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;Department of Tumor Pathology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;First Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi Ward, 431-3192, Hamamatsu, Shizuoka, Japan;

关键词: Lung cancer;    Cigarette smoking;    Recurrence risk;    Lipid biomarker;    Mass spectrometry;   

DOI  :  10.1186/s12944-023-01778-3

 received in 2023-01-04, accepted in 2023-01-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe risk of postoperative recurrence is higher in lung cancer patients who smoke than non-smokers. However, objective evaluation of the postoperative recurrence risk is difficult using conventional pathological prognostic factors because of their lack of reproducibility. Consequently, novel objective biomarkers that reflect postoperative risk in lung cancer patients who smoke must be identified. Because cigarette smoking and oncogenesis alter lipid metabolism in lung tissue, we hypothesized that the lipid profiles in lung cancer tissues are influenced by cigarette smoking and can reflect the postoperative recurrence risk in smoking lung cancer patients. This study aimed to identify lipid biomarkers that reflect the smoking status and the postoperative recurrence risk.MethodsPrimary tumor tissues of lung adenocarcinoma (ADC) (n = 26) and squamous cell carcinoma (SQCC) (n = 18) obtained from surgery were assigned to subgroups according to the patient’s smoking status. The ADC cohort was divided into never smoker and smoker groups, while the SQCC cohort was divided into moderate smoker and heavy smoker groups. Extracted lipids from the tumor tissues were subjected to liquid chromatography-tandem mass spectrometry analysis. Lipids that were influenced by smoking status and reflected postoperative recurrence and pathological prognostic factors were screened.ResultsTwo and 12 lipid peaks in the ADC and SQCC cohorts showed a significant positive correlation with the Brinkman index, respectively. Among them, in the ADC cohort, a higher lipid level consisted of three phosphatidylcholine (PC) isomers, PC (14:0_18:2), PC (16:1_16:1), and PC (16:0_16:2), was associated with a shorter recurrence free period (RFP) and a greater likelihoods of progressed T-factor (≥ pT2) and pleural invasion. In the SQCC cohort, a lower m/z 736.5276 level was associated with shorter RFP and greater likelihood of recurrence.ConclusionsFrom our data, we propose three PC isomers, PC (14:0_18:2), PC (16:1_16:1), and PC (16:0_16:2), and a lipid peak of m/z 736.5276 as novel candidate biomarkers for postoperative recurrence risk in lung ADC and SQCC patients who are smokers.

【 授权许可】

CC BY   
© The Author(s) 2023

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