Honey proteins regulate oxidative stress, inflammation and ameliorates hyperglycemia in streptozotocin induced diabetic rats

BMC Complementary Medicine and Therapies

Research

Nida Dastagir¹  Almas Jabeen²  Farwa Naqvi² 

[1] Dow University of Health Sciences (DUHS), Karachi, Pakistan;Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270, Karachi, Pakistan;

关键词: Diabetes;    Inflammation;    Honey Proteins;    Immunomodulatory;    Ziziphus;   

DOI  :  10.1186/s12906-023-03837-9

 received in 2022-09-01, accepted in 2023-01-06,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundDiabetes Mellitus (DM) poses a serious health problem worldwide and several inflammatory mediators are involved in the pathogenesis of this disease. Honey composed of various constituents which have been proven to have immunomodulatory and anti-inflammatory properties. The aim of this study is to investigate the in vitro and in vivo effects of Ziziphus honey and its isolated crude proteins in modulation of immune system and inflammation involved in the pathogenesis of diabetes.MethodologyThe proteins from Ziziphus honey were isolated by ammonium sulfate precipitation and estimated by Bradford method. In vitro anti-inflammatory activities were evaluated by inhibition of reactive oxygen species (ROS) from phagocytes via chemiluminescence immunoassay and nitric oxide (NO) by Griess method. Cytotoxicity was evaluated by MTT Assay. The comparative effect of oral and IP routes of honey and isolated proteins was observed in streptozotocin (STZ) induced diabetic male Wistar rats. qRT-PCR technique was utilized for gene expression studies.ResultsThe honey proteins suppressed phagocyte oxidative burst and nitric oxide (NO) at significantly lower concentrations as compared to crude honey. The isolated proteins showed promising anti-inflammatory and hypoglycemic effects along with maintenance of body weight of rodents via both oral and IP routes, with significant down-regulation of inflammatory markers TNF-α, IL-1β, IFN-γ, iNOS, caspase 1, Calgranulin A (S100A8) and NF-κB expression in diabetic rats.ConclusionThe isolated honey proteins showed better immunomodulatory and therapeutic potential at significantly lower doses as compared to crude honey.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 1

41116_2022_35_Article_IEq435.gif

41116_2022_35_Article_IEq433.gif

12888_2022_4443_Article_IEq12.gif

12888_2022_4443_Article_IEq10.gif

12888_2022_4443_Article_IEq8.gif

41116_2022_35_Article_IEq425.gif

12888_2022_4443_Article_IEq6.gif

41116_2022_35_Article_IEq420.gif

41116_2022_35_Article_IEq416.gif

41116_2022_35_Article_IEq409.gif

41116_2022_35_Article_IEq404.gif