Periostin overexpression in scleroderma cardiac tissue and its utility as a marker for disease complications

Arthritis Research & Therapy

Research

Mortada Najem¹  Maria Trojanowska²  Giuseppina Farina²  Andreea M. Bujor²  Fatima El-Adili³  Justin K. Lui⁴  Flora Sam⁵ 

[1] Division of Rheumatology, Boston University School of Medicine, Boston, MA, USA;Division of Rheumatology, Boston University School of Medicine, Boston, MA, USA;Arthritis and Autoimmune Diseases Center, Boston University, 72 E Concord St, Evans 501, 02118, Boston, MA, USA;Division of Rheumatology, Boston University School of Medicine, Boston, MA, USA;Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA, USA;Arthritis and Autoimmune Diseases Center, Boston University, 72 E Concord St, Evans 501, 02118, Boston, MA, USA;The Pulmonary Center, Boston University School of Medicine, Boston, MA, USA;Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA, USA;

关键词: Scleroderma;    Periostin;    Biomarkers;    Fibrosis;   

DOI  :  10.1186/s13075-022-02943-2

 received in 2022-03-24, accepted in 2022-11-02,  发布年份 2022

来源: Springer

【 摘 要 】

ObjectiveTo evaluate the levels of periostin in patients with systemic sclerosis (SSc) and their association with features of systemic sclerosis.MethodsThe levels of periostin were assessed in the serum of 106 SSc patients and 22 healthy controls and by immunofluorescence staining in cardiac tissue from 4 SSc patients and 4 controls. Serum periostin was measured via enzyme-linked immunosorbent assay. The results were analyzed using Mann-Whitney test or Kruskal-Wallis test followed by Dunn’s multiple comparisons tests and Spearman’s test for correlations. Cardiac tissue from SSc patients and controls was stained for periostin and co-stained for periostin and collagen type I using immunofluorescence.ResultsPeriostin levels were higher in patients with SSc compared to controls and directly correlated to modified Rodnan skin score and echocardiography parameters of left ventricular measurements. Immunofluorescence staining in SSc cardiac tissue showed patchy periostin expression in all SSc patients, but not in controls. Furthermore, there was extensive periostin expression even in areas without collagen deposition, while all established fibrotic areas showed colocalization of collagen and periostin. There was no association between periostin levels and interstitial lung disease, pulmonary hypertension or other vascular complications.ConclusionPeriostin is elevated in SSc cardiac tissue in vivo and circulating levels of periostin are increased in SSc, correlating with the extent of disease duration, degree of skin fibrosis, and left ventricular structural assessments. Periostin may be a potential biomarker that can provide further pathogenic insight into cardiac fibrosis in SSc.

【 授权许可】

CC BY   
© The Author(s) 2022. corrected publication 2023

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Fig. 14