Synaptotagmin-like protein 1 is a potential diagnostic and prognostic biomarker in endometrial cancer based on bioinformatics and experiments

Journal of Ovarian Research

Research

Xu Meng¹  Cai Meijuan²  Wang Qian²  Liu Fang³ 

[1] Department of Clinical Laboratory, Qilu Hospital of Shandong University (Qingdao), Qingdao, Shandong, China;Department of Clinical Laboratory, Qilu Hospital of Shandong University, No.107 Wenhua West Road, 250013, Jinan, Shandong, China;Department of Clinical Laboratory, Qilu Hospital of Shandong University (Qingdao), Qingdao, Shandong, China;Department of Pathology, Qingdao Chengyang People’s Hospital, No.758 Hefei Road, 266035, Qingdao, Shandong, China;

关键词: Endometrial cancer;    Synaptotagmin-like protein 1;    Prognosis;    DNA methylation;    Immune infiltration;   

DOI  :  10.1186/s13048-023-01097-2

 received in 2022-03-23, accepted in 2023-01-11,  发布年份 2023

来源: Springer

【 摘 要 】

Endometrial cancer (EC) is one of the most common gynecologic malignancies. Identification of potential EC biomarkers is essential to improve the prognosis and development of therapies against EC. Synaptotagmin-like protein 1 (SYTL1), as a small GTPase Rab27 effector, mainly plays a role in vesicle trafficking and cytotoxic granule exocytosis in lymphocytes. However the role of SYTL1 in EC remains uncertain. We performed a comprehensive assessment of the relationship between SYTL1 and patient diagnosis and prognosis by analysis of EC patients’ data from TCGA. We employed the LinkedOmics and Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database to analyze the biological function of SYTL1 in EC. In addition, the correlation between SYTL1 expression and its DNA methylation was performed by using cBioportal, UALCAN, TCGA Wanderer and MethSurv databases. We further assessed the link between SYTL1 and tumor-infiltrating immune cells by using gene set variation analysis (GSVA).Results We found that SYTL1 was highly expressed in EC patients and cell lines. And increased expression of SYTL1 was associated with age, clinical stage, histological type, histological grade and good overall survival (OS).SYTL1 DNA methylation is negatively associated with SYTL1 expression and UCEC patients’ OS. SYTL1 expression is closely correlated with immune infiltration. Furthermore, we carried out in vitro experiments to verify the results of bioinformatic analysis.Conclusion Our results demonstrated that the elevation of SYTL1 expression is associated with good OS and SYTL1 might be a potential diagnostic and prognostic marker in EC.

【 授权许可】

CC BY   
© The Author(s) 2023

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