Ginkgolide B inhibits lung cancer cells promotion via beclin-1-dependent autophagy

BMC Complementary Medicine and Therapies

Research Article

Wen-Qiang Quan¹  Yi-Wen Yao¹  Zu-Jun Sun¹  Ping Ji¹  Jun-Lu Wu¹  Dong Li¹  Xuan Wang²  Hao Zhou²  Qi-Hui Shao³ 

[1] Department of Clinical Laboratory, Shanghai Tongji Hospital, Tongji University School of Medicine, 200065, Shanghai, China;Department of Pharmacy, Putuo People’s Hospital, 200060, Shanghai, China;Department of Traditional Chinese Medicine, Shanghai Tongji Hospital, Tongji University School of Medicine, 200065, Shanghai, China;

关键词: Ginkgolide B;    Light chain 3B;    NLRP3;    Beclin-1;    Autophagy;   

DOI  :  10.1186/s12906-020-02980-x

 received in 2020-02-17, accepted in 2020-06-01,  发布年份 2020

来源: Springer

【 摘 要 】

BackgroundGinkgolide B (GKB) is a major active component of the extracts of Ginkgo biloba leaves, and it has been used as an anti-cancer agent. However, it is unknown whether GKB has the therapeutic effects on lung cancer. Here, we studied the effects of GKB on lung cancer cells.MethodsThe effects of GKB on lung cancer cell proliferation and invasion were analyzed by cell counting kit (CCK-8) and cell invasion assays, respectively. Apoptosis was detected by flow cytometry. Western blot analysis was used to confirm the expression of autophagy-associated proteins in GKB-treated cells. Immunofluorescence analysis was used to analyze the level of light chain 3B (LC3B).ResultsTreatment with GKB time-dependently inhibited the proliferation and decreased the invasive capacity of A549 and H1975 cells. GKB induced apoptosis of these cells, but there was no significant effect on apoptosis compared to the control treatment. GKB-induced inhibition of cell proliferation and GKB-induced cell death were due to autophagy rather than apoptosis. GKB-induced autophagy of lung cancer cells was dependent on beclin-1, and autophagy-induced inhibition of the NLRP3 inflammasome contributed to the anti-tumor effect of GKB.ConclusionsGKB-mediated autophagy of lung cancer cells is beclin-1-dependent and results in inhibition of the NLRP3 inflammasome. Therefore, GKB might be a potential therapeutic candidate for the treatment of lung cancer.

【 授权许可】

CC BY   
© The Author(s). 2020

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