The transcriptomic profile of Spodoptera frugiperda differs in response to a novel insecticide, cyproflanilide, compared to chlorantraniliprole and avermectin

BMC Genomics

Research

Zhengbing Zhang¹  Yufeng Lin¹  Lin Qiu²  Haijuan Shu²  Hualiang He²  Jin Xue²  Qiao Gao²  Youzhi Li³  Wenbing Ding³ 

[1] Agriculture and Rural Department of Hunan Province, Plant Protection and Inspection Station, 410005, Changsha, China;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, 410128, Changsha, China;Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, 410128, Changsha, China;National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, 410128, Changsha, China;

关键词: Cyproflanilide;    Spodoptera frugiperda;    Transcriptomic analyses;    GABAR;    P450;   

DOI  :  10.1186/s12864-022-09095-2

 received in 2022-08-31, accepted in 2022-12-22,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundCyproflanilide is a novel chemical that is already undergoing insecticide registration in China and has been categorized as a member of group 30 by the IRAC. Since it was first detected in 2019, the fall armyworm (FAW), Spodoptera frugiperda, has become a serious pest in China. Our laboratory and field efficacy trials indicated that cyproflanilide exhibits high larvicidal activity against FAW. However, the effect of cyproflanilide against FAW remains unknown. And it is worth exploring further before the cyproflanilide becomes commercially available.ResultsWe found larvae exposed to cyproflanilide had significantly shorter body length and higher death rates compared to control larvae. Additionally, we found surviving larvae had a significantly longer developmental period compared to control larvae. The potential molecular mechanisms of cyproflanilide against FAW were investigated using comparative transcriptomic analyses on larval samples subjected to three insecticide treatments, including cyproflanilide and two other commonly used insecticides against FAW in China, chlorantraniliprole and avermectin. We found that several subunits of the γ-aminobutyric acid receptor (GABAR), a possible target protein of cyproflanilide, were significantly up-regulated at the transcriptional level during cyproflanilide-induced stress. Additionally, between the control and cyproflanilide-treated samples, we identified 131 differentially expressed genes (DEGs) associated with detoxification metabolism. Of these, we found four P450 genes that were significantly up-regulated under cyproflanilide stress but were not DEGs when exposed to chlorantraniliprole and avermectin, or 23 other pesticides from previous reports. Furthermore, we discovered an interesting gene aggregation region for insect cuticle proteins (CPs) on the 18th chromosome, which is likely related to FAW cross-resistance to cyproflanilide and avermectin.ConclusionsOur results contribute to a greater understanding of the mechanisms by which cyproflanilide affects FAW. Additionally, we identified the similarities and differences in transcriptomic profiling of FAW between the novel insecticide cyproflanilide and two other commonly used insecticides.

【 授权许可】

CC BY   
© The Author(s) 2022

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