Characterization of the bacterial microbiome of Swedish ticks through 16S rRNA amplicon sequencing of whole ticks and of individual tick organs

Parasites & Vectors

Research

Davide Sassera¹  Per-Eric Lindgren²  Anna Omazic³  Giulia Chiappa⁴  Karin Ullman⁴  Emma Östlund⁴  Debora Perissinotto⁴  Robert Söderlund⁴  Giulio Grandi⁵  Emanuela Olivieri⁶ 

[1] Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Pavia, Italy;Department of Biomedical and Clinical Sciences, Division of Inflammation and Infection, Linköping University, 581 85, Linköping, Sweden;Department of Clinical Microbiology, County Hospital Ryhov, 551 85, Jönköping, Sweden;Department of Chemistry, Environment, and Feed Hygiene, National Veterinary Institute (SVA), 751 89, Uppsala, Sweden;Department of Microbiology, National Veterinary Institute (SVA), 751 89, Uppsala, Sweden;Department of Microbiology, National Veterinary Institute (SVA), 751 89, Uppsala, Sweden;Department of Biomedical Sciences and Veterinary Public Health (BVF), Swedish University of Agricultural Sciences (SLU), Ulls Väg 26, 750 07, Uppsala, Sweden;Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Strada Campeggi, 59/61, 27100, Pavia, Italy;

关键词: Ixodes ricinus;    Microbiota;    Community profiling;    Tick-borne pathogens;    One Health;    NGS;    16S;    Endosymbiont;    Borrelia;    Midichloria;   

DOI  :  10.1186/s13071-022-05638-4

 received in 2022-11-22, accepted in 2022-12-24,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe composition of the microbial flora associated with ixodid ticks has been studied in several species, revealing the importance of geographical origin, developmental stage(s) and feeding status of the tick, as well as substantial differences between tissues and organs. Studying the microbiome in the correct context and scale is therefore necessary for understanding the interactions between tick-borne pathogens and other microorganisms as well as other aspects of tick biology.MethodsIn the present study the microbial flora of whole Ixodes ricinus, I. persulcatus and I. trianguliceps ticks were analyzed with 16S rRNA amplicon sequencing. Additionally, tick organs (midguts, Malpighian tubules, ovaries, salivary glands) from flat and engorged I. ricinus female ticks were examined with the same methodology.ResultsThe most abundant bacteria belonged to the group of Proteobacteria (Cand. Midichloria mitochondrii and Cand. Lariskella). 16S amplicon sequencing of dissected tick organs provided more information on the diversity of I. ricinus-associated microbial flora, especially when organs were collected from engorged ticks. Bacterial genera significantly associated with tick feeding status as well as genera associated with the presence of tick-borne pathogens were identified.ConclusionsThese results contribute to the knowledge of microbial flora associated with ixodid ticks in their northernmost distribution limit in Europe and opens new perspectives for other investigations on the function of these bacteria, including those using other approaches like in vitro cultivation and in vitro models.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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