Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factor

BMC Plant Biology

Research

Canbin Chen¹  Jiaxuan Chen¹  Jietang Zhao¹  Jianye Chen¹  Qingzhu Hua¹  Yonghua Qin¹  Kamran Shah¹  Zhike Zhang¹  Jiayi Chen¹  Guibing Hu¹  Fangfang Xie² 

[1] Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, 510642, Guangzhou, China;Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, 510642, Guangzhou, China;College of Agriculture, Guangxi University, 530004, Nanning, China;

关键词: Betalain biosynthesis;    MYB transcription factor;    Pitaya;    Transcription activity;   

DOI  :  10.1186/s12870-023-04049-6

 received in 2022-07-19, accepted in 2023-01-06,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundMultiple MYB transcription factors (TFs) are involved in the regulation of plant coloring. Betalain is a kind of natural plant pigment and its biosynthesis is regulated by a number of enzymes. Despite this, little is known about the molecular properties and roles of MYB TFs in pitaya betalain biosynthesis.ResultsIn the present study, we identified a 1R-MYB gene, HuMYB132, which is preferentially expressed in red-pulp pitaya at the mature stage. It was clustered with Arabidopsis R-R-type genes and had two DNA-binding domains and a histidine-rich region. The expression assays in N. benthamiana and yeast indicated that HuMYB132 is a nucleus-localized protein with transcriptional activation activity. Dual luciferase reporter assay and electrophoretic mobility shift assays (EMSA) demonstrated that HuMYB132 could promote the transcriptional activities of HuADH1, HuCYP76AD1–1, and HuDODA1 by binding to their promoters. Silencing HuMYB132 reduced betalain accumulation and the expression levels of betalain biosynthetic genes in pitaya pulps.ConclusionsAccording to our findings, HuMYB132, a R-R type member of 1R-MYB TF subfamily, positively regulates pitaya betalain biosynthesis by regulating the expression of HuADH1, HuCYP76AD1–1, and HuDODA1. The present study provides a new theoretical reference for the management of pitaya betalain biosynthesis and also provides an essential basis for future regulation of betalain biosynthesis in Hylocereus.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

41116_2022_35_Article_IEq281.gif

41116_2022_35_Article_IEq279.gif

41116_2022_35_Article_IEq273.gif

41116_2022_35_Article_IEq268.gif

41116_2022_35_Article_IEq257.gif

41116_2022_35_Article_IEq253.gif

41116_2022_35_Article_IEq241.gif

41116_2022_35_Article_IEq230.gif

41116_2022_35_Article_IEq210.gif

41116_2022_35_Article_IEq196.gif