Differential transcriptome analysis of Sporocytophaga sp. CX11 and identification of candidate genes involved in lignocellulose degradation

Bioresources and Bioprocessing

Research

Xiangyi Wang¹  Xu Lin¹  Fan Yang¹  Xiaoyi Chen¹  Ying Zhuang¹  Xianghe Song¹  Jiwei Wang¹ 

[1] School of Biological Engineering, Dalian Polytechnic University, Ganjingziqu, 116034, Dalian, People’s Republic of China;

关键词: Cellulose biodegradation;    Sporocytophaga;    Differential transcriptome analysis;    Carbohydrate-active enzymes;    Gliding motility;   

DOI  :  10.1186/s40643-023-00629-4

 received in 2022-10-09, accepted in 2023-01-12,  发布年份 2023

来源: Springer

【 摘 要 】

Cellulose is the most abundant renewable bioresources on earth, and the biodegradation and utilization of cellulose would contribute to the sustainable development of global environment. Sporocytophaga species are common aerobic cellulose-degrading bacteria in soil, which can adhere to the surface of cellulose matrix and motile by gliding. In this study, a differential transcriptome analysis of Sporocytophaga sp. CX11 was performed and a total of 4,217 differentially expressed genes (DEGs) were identified. Gene Ontology enrichment results showed that there are three GO categories related to cellulose degradation function among the annotated DEGs. A total of 177 DEGs were identified as genes encoding carbohydrate-active enzymes (CAZymes), among which 54 significantly upregulated CAZymes were mainly cellulases, hemicellulases, pectinases, etc. 39 DEGs were screened to associate with gliding function. In order to explore unannotated genes potentially related to cellulose metabolism, cluster analysis was performed using the Short-Time Series Expression Miner algorithm (STEM). 281 unannotated genes were predicted to be associated with the initial-middle stage of cellulose degradation and 289 unannotated genes might function in the middle-last stage of cellulose degradation. Sporocytophaga sp. CX11 could produce extracellular endo-xylanase, endo-glucanase, FPase and β-glucosidase, respectively, according to different carbon source conditions. Altogether, this study provides valuable insights into the transcriptome information of Sporocytophaga sp. CX11, which would be useful to explore its application in biodegradation and utilization of cellulose resources.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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