Anthocyanin metabolism in Nelumbo: translational and post-translational regulation control transcription

BMC Plant Biology

Research

Wen chen¹  Xiaojing Liu²  Fengfeng Du²  Jian cui²  Linhe Sun²  Dongrui Yao²  Shaozhou Chen²  Naiwei Li²  Yajun Chang²  Jinfeng Li² 

[1] Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, 311300, Hangzhou, Zhejiang, China;Jiangsu Key Laboratory for the Research and Utilization of Plant Resources/Jiangsu Engineering Research Center of Aquatic Plant Resources and Water Environment Remediation, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, 210014, Nanjing, China;

关键词: Anthocyanin;    Proanthocyanidins;    Nelumbo;    Metabolic flux;    Lotus;   

DOI  :  10.1186/s12870-023-04068-3

 received in 2022-11-04, accepted in 2023-01-16,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundLotus (Nelumbo Adans.) is used as an herbal medicine and the flowers are a source of natural flavonoids. ‘Da Sajin’, which was firstly found in the plateau area, is a natural mutant in flower color with red streamers dyeing around white petals.ResultsThe LC–MS-MS results showed that eight anthocyanin compounds, including cyanidin 3-O-glucoside, cyanidin 3-O-galactoside, malvidin 3-O-galactoside, and malvidin 3-O-glucoside, were differentially enriched in red-pigmented tissues of the petals, whereas most of these metabolites were undetected in white tissues of the petals. Transcriptome profiling indicated that the relative high expression levels of structural genes, such as NnPAL, NnF3H, and NnANS, was inconsistent with the low anthocyanin concentration in white tissues. Members of the NnMYB and NnbHLH transcription factor families were presumed to play a role in the metabolic flux in the anthocyanin and proanthocyanidin biosynthetic pathway. The expression model of translational initiation factor, ribosomal proteins and SKP1–CUL1–F-box protein complex related genes suggested an important role for translational and post-translational network in anthocyanin biosynthesis. In addition, pathway analysis indicated that light reaction or photo destruction might be an important external cause for floral color determination in lotus.ConclusionsIn this study, it is supposed that the natural lotus mutant ‘Da Sajin’ may have originated from a red-flowered ancestor. Partial loss of anthocyanin pigments in petals may result from metabolic disorder caused by light destruction. This disorder is mainly regulated at post translation and translation level, resulting in a non-inherited phenotype. These results contribute to an improved understanding of anthocyanin metabolism in lotus, and indicate that the translational and post-translational regulatory network determines the metabolic flux of anthocyanins and proanthocyanidins under specific environmental conditions.

【 授权许可】

CC BY   
© The Author(s) 2023

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