Comparative genetic characterization of CMY-2-type beta-lactamase producing pathogenic Escherichia coli isolated from humans and pigs suffering from diarrhea in Korea

Annals of Clinical Microbiology and Antimicrobials

Research

Kwang-Won Seo¹  Woo-Kon Lee²  Min-Kyoung Shin²  Wan-Kyu Lee³  Kyung-Hyo Do³ 

[1] College of Veterinary Medicine, Chungbuk National University, 28644, Cheongju, Republic of Korea;Department of Microbiology, College of Medicine, Gyeongsang National University, 52727, Jinju, Republic of Korea;Laboratory of Veterinary Bacteriology and Infectious Diseases, College of Veterinary Medicine, Chungbuk National University, 28644, Cheongju, Republic of Korea;

关键词: Escherichia coli;    Pig;    Humans;    Antimicrobial resistance;    Third-generation cephalosporin;    Plasmid-mediated AmpC;   

DOI  :  10.1186/s12941-023-00559-1

 received in 2022-08-09, accepted in 2023-01-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundPathogenic Escherichia coli are an important cause of bacterial infections in both humans and pigs and many of antimicrobials are used for the treatment of E. coli infection. The objective of this study was to investigate the characteristics and relationship between humans and pigs regarding third-generation cephalosporin resistance and CMY-2-producing E. coli in Korea.ResultsAll 103 third-generation cephalosporin-resistant E. coli isolates showed multidrug resistance. Also, except for β-lactam/β-lactamase inhibitor combinations, all antimicrobials resistant rates were higher in pigs than in humans. A total of 36 isolates (humans: five isolates; pigs: 31 isolates) were positive for the CMY-2-encoding genes and thirty-two (88.9%) isolates detected class 1 integrons with 10 different gene cassette arrangements, and only 1 isolate detected a class 2 integron. The most common virulence genes in pigs were LT (71.0%), F18 (51.6%), and STb (51.6%), while stx2 (80.0%) was the most frequently detected gene in humans. Stx2 gene was also detected in pigs (6.5%). Interestingly, 36 CMY-2-producing E. coli isolates showed a high diversity of sequence types (ST), and ST88 was present in E. coli from both pigs (11 isolates) and humans (one isolate).ConclusionOur findings suggest that a critical need for comprehensive surveillance of third-generation cephalosporin resistance is necessary to preserve the usefulness of third-generation cephalosporins in both humans and pigs.

【 授权许可】

CC BY   
© The Author(s) 2023

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