Multiple variation patterns of terpene synthases in 26 maize genomes

BMC Genomics

Research

Xiang-dong Kong¹  Li-na Xu²  Wen-hui Ma³  Ping Ren³  Si-xian Liu³  Wenqing Xiao³  Yang Sun⁴  Jing Wang⁴  Qing-nan Wang⁵  Yong-Jun Zhang⁶ 

[1] Institute of Bioinformatics, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China;Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, 230031, Hefei, China;Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, 241000, Wuhu, Anhui, China;Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, 241000, Wuhu, Anhui, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, 241000, Wuhu, Anhui, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, 230031, Hefei, China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;

关键词: Terpene synthases;    Pangenome;    Positive selection;    Structural variation;    Atypical;    Ostrinia furnacalis;   

DOI  :  10.1186/s12864-023-09137-3

 received in 2022-09-28, accepted in 2023-01-13,  发布年份 2023

来源: Springer

【 摘 要 】

Terpenoids are important compounds associated with the pest and herbivore resistance mechanisms of plants; consequently, it is essential to identify and explore terpene synthase (TPS) genes in maize. In the present study, we identified 31 TPS genes based on a pan-genome of 26 high-quality maize genomes containing 20 core genes (present in all 26 lines), seven dispensable genes (present in 2 to 23 lines), three near-core genes (present in 24 to 25 lines), and one private gene (present in only 1 line). Evaluation of ka/ks values of TPS in 26 varieties revealed that TPS25 was subjected to positive selection in some varieties. Six ZmTPS had ka/ks values less than 1, indicating that they were subjected to purifying selection. In 26 genomes, significant differences were observed in ZmTPS25 expression between genes affected by structural variation (SV) and those not affected by SV. In some varieties, SV altered the conserved structural domains resulting in a considerable number of atypical genes. The analysis of RNA-seq data of maize Ostrinia furnacalis feeding revealed 10 differentially expressed ZmTPS, 9 of which were core genes. However, many atypical genes for these responsive genes were identified in several genomes. These findings provide a novel resource for functional studies of ZmTPS.

【 授权许可】

CC BY   
© The Author(s) 2023

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