RETRACTED ARTICLE: CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p

Molecular Cancer

Research

Shuli Zhao¹  Hongyong Cao²  Dawei Rong²  Weiwei Tang²  Kai Fu²  Chen Lu²  Betty Zhang³ 

[1] Department of General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China;Department of General Surgery, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China;Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario, Canada;

关键词: Gastric cancer;    circPSMC3;    miR-296-5p;    PTEN;    Therapy;   

DOI  :  10.1186/s12943-019-0958-6

 received in 2018-09-29, accepted in 2019-02-13,  发布年份 2019

来源: Springer

【 摘 要 】

BackgroundCircular RNAs (circRNAs) are a class of non-coding RNAs with a loop structure, but its functions remain largely unknown. Growing evidence has revealed that circRNAs play a striking role as functional RNAs in the progression of malignant disease. However, the precise role of circRNAs in gastric cancer (GC) remains unclear.MethodsCircRNAs were determined by human circRNA array analysis and quantitative reverse transcription polymerase reaction. Luciferase reporter, RNA pull down, and fluorescence in situ hybridization assays were employed to test the interaction between circPSMC3 and miR-296-5p. Ectopic over-expression and siRNA-mediated knockdown of circPSMC3, proliferation, migration and invasion in vitro, and in vivo experiment of metastasis were used to evaluate the function of circPSMC3.ResultsCircPSMC3 rather than liner PSMC3 mRNA was down-regulated in GC tissues, corresponding plasmas from GC patients as well as GC cell lines compared to normal controls. Lower circPSMC3 expression in GC patients was correlated with higher TNM stage and shorter overall survival. Over-expression of circPSMC3 and miR-296-5p inhibitor could inhibit the tumorigenesis of gastric cancer cells in vivo and vitro whereas co-transfection of circPSMC3 and miRNA-296-5p could counteract this effect. Importantly, we demonstrated that circPSMC3 could act as a sponge of miR-296-5p to regulate the expression of Phosphatase and Tensin Homolog (PTEN), and further suppress the tumorigenesis of gastric cancer cells.ConclusionOur study reveals that circPSMC3 can serve as a novel potential circulating biomarker for detection of GC. CircPSMC3 participates in progression of gastric cancer by sponging miRNA-296-5p with PTEN, providing a new insight into the treatment of gastric cancer.

【 授权许可】

CC BY   
© The Author(s). 2019

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