Resveratrol ameliorates bisphenol A-induced testicular toxicity in adult male rats: a stereological and functional study

Basic and Clinical Andrology

Research Article

Elham Aliabadi¹  Maryam Naseh²  Hossein Bordbar³  Seyedeh-Saeedeh Yahyavi³  Ali Noorafshan³ 

[1] Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Zand Ave., 71348-45794, Shiraz, Iran;Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Zand Ave., 71348-45794, Shiraz, Iran;Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;

关键词: Bisphenol A;    Resveratrol;    Testicular toxicity;    Sperm parameters;    Stereology;   

DOI  :  10.1186/s12610-022-00174-8

 received in 2022-07-25, accepted in 2022-10-24,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundBisphenol A (BPA) is one of the most widely used synthetic chemicals worldwide. BPA as an endocrine disruptor affects the reproductive systems through estrogenic and antiandrogenic proprieties. Resveratrol (RES) as a natural polyphenol and potent antioxidant exhibits protective effects against reproductive toxicity by inhibiting of oxidative stress. 48 male rats were divided into eight groups (n=6), including CONTROL, OLIVE OIL (0.5 ml/ day), Carboxy methylcellulose (CMC) (1 ml of 10 g/l), RES (100mg/kg/day), low dose of BPA (25 mg/kg/day), high dose of BPA (50 mg/kg/day), low dose of BPA + RES, and high dose of BPA + RES. All treatments were done orally per day for 56 days. At the end of the 8th week, blood samples were collected for hormone assays. Then, the sperm parameters were analyzed, and the left testis was removed for stereological study.ResultsWe showed a significant decrease in sperm parameters in the low and high doses of BPA groups compared to control groups (P<0.05). The volume of testicular components as well as the diameter and length of seminiferous tubules significantly reduced (11-64 %), and the total number of the testicular cell types decreased (34-67 %) on average in the low and high doses of BPA groups. Moreover, serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone hormones concentration showed a significant reduction in both doses of BPA groups (P<0.01). Nonetheless, treatment with RES could ameliorate all the above-mentioned changes in the low and high doses of BPA groups (P<0.05).ConclusionsRES could prevent BPA-induced testicular structural changes and sperm quality via improving gonadotropin hormones and testosterone levels.

【 授权许可】

CC BY   
© The Author(s) 2022

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