Identification of immune associated potential molecular targets in proliferative diabetic retinopathy

BMC Ophthalmology

Research

Ying Gao¹  Feng Yan¹  Changlin Zhao¹  Yun Tang¹  Jingyu Liu¹  Yirui Ge¹  Hu Meng¹  Xiaomin Zhu¹  Yan Lu¹  Min Xue²  Bing Dai³ 

[1] Department of Ophthalmology, Affilia Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu Province, China;Department of Ophthalmology, Anhui NO.2 Provincial People’s Hospital, Hefei, Anhui, China;Department of Vascular Surgery, Tianjin Hospital, Tianjin, China;

关键词: Proliferative diabetic retinopathy;    Neovascular membrane;    Immune;    Inflammation;    Genes;    Biomarkers;   

DOI  :  10.1186/s12886-023-02774-y

 received in 2022-03-15, accepted in 2023-01-06,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundDiabetic retinopathy (DR) is one of the most common microvascular complications of diabetes and causes of blindness in developed countries. Our study was designed to identify immune-related genes involved in the progression of proliferative diabetic retinopathy (PDR).MethodsThe “GSE102485” dataset of neovascular membrane samples (NVMs) from type 1 and 2 diabetes mellitus patients was downloaded from the Gene Expression Omnibus database. Functional enrichment analyses, protein–protein interaction network (PPI) construction, and module analysis of immune pathways in NVMs and controls were conducted via Gene Set Enrichment Analysis and Metascape.ResultsThe significantly upregulated hallmark gene sets in DR2 and DR1 groups were involved in five immune pathways. Only CCR4, CXCR6, C3AR1, LPAR1, C5AR1, and P2RY14 were not previously reported in the context of PDR molecular pathophysiology. Except for P2RY14, all of the above were upregulated in retinal samples from experimental diabetes mouse models and human retina microvascular endothelial cells (HRMECs) treated with high glucose (HG) by quantitative Real Time Polymerase Chain Reaction (qRT-PCR).ConclusionThe genes identified herein provide insight into immune-related differential gene expression during DR progression.

【 授权许可】

CC BY   
© The Author(s) 2023

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