Multi-omics analysis of sarcospan overexpression in mdx skeletal muscle reveals compensatory remodeling of cytoskeleton-matrix interactions that promote mechanotransduction pathways

Skeletal Muscle

Research

Lauren R. Schmitt¹  Kirk C. Hansen¹  Yerbol Z. Kurmangaliyev²  Cynthia Shu³  Elizabeth M. Gibbs³  Jackie L. McCourt³  Hafsa Mamsa³  Mohammad Hossein Afsharinia³  Pranav Kannan³  Kara M. Shin³  Rachelle H. Crosbie⁴  Kristen M. Stearns-Reider⁵ 

[1] Department of Biochemistry and Molecular Genetics, University of Colorado, Denver, CO, USA;Department of Biological Chemistry, Howard Hughes Medical Institute, David Geffen School of Medicine, University of California, Los Angeles, CA, USA;Department of Integrative Biology and Physiology, University of California, 90095, Los Angeles, CA, USA;Department of Integrative Biology and Physiology, University of California, 90095, Los Angeles, CA, USA;Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA;Molecular Biology Institute, University of California, Los Angeles, CA, USA;Broad Stem Cell Research Center, University of California, Los Angeles, CA, USA;Department of Integrative Biology and Physiology, University of California, 90095, Los Angeles, CA, USA;Department of Orthopedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA, USA;

关键词: Duchenne muscular dystrophy;    Dystrophin;    Dystroglycan;    Extracellular matrix;    Sarcospan;   

DOI  :  10.1186/s13395-022-00311-x

 received in 2022-07-09, accepted in 2022-12-06,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe dystrophin-glycoprotein complex (DGC) is a critical adhesion complex of the muscle cell membrane, providing a mechanical link between the extracellular matrix (ECM) and the cortical cytoskeleton that stabilizes the sarcolemma during repeated muscle contractions. One integral component of the DGC is the transmembrane protein, sarcospan (SSPN). Overexpression of SSPN in the skeletal muscle of mdx mice (murine model of DMD) restores muscle fiber attachment to the ECM in part through an associated increase in utrophin and integrin adhesion complexes at the cell membrane, protecting the muscle from contraction-induced injury. In this study, we utilized transcriptomic and ECM protein-optimized proteomics data sets from wild-type, mdx, and mdx transgenic (mdxTG) skeletal muscle tissues to identify pathways and proteins driving the compensatory action of SSPN overexpression.MethodsThe tibialis anterior and quadriceps muscles were isolated from wild-type, mdx, and mdxTG mice and subjected to bulk RNA-Seq and global proteomics analysis using methods to enhance capture of ECM proteins. Data sets were further analyzed through the ingenuity pathway analysis (QIAGEN) and integrative gene set enrichment to identify candidate networks, signaling pathways, and upstream regulators.ResultsThrough our multi-omics approach, we identified 3 classes of differentially expressed genes and proteins in mdxTG muscle, including those that were (1) unrestored (significantly different from wild type, but not from mdx), (2) restored (significantly different from mdx, but not from wild type), and (3) compensatory (significantly different from both wild type and mdx). We identified signaling pathways that may contribute to the rescue phenotype, most notably cytoskeleton and ECM organization pathways. ECM-optimized proteomics revealed an increased abundance of collagens II, V, and XI, along with β-spectrin in mdxTG samples. Using ingenuity pathway analysis, we identified upstream regulators that are computationally predicted to drive compensatory changes, revealing a possible mechanism of SSPN rescue through a rewiring of cell-ECM bidirectional communication. We found that SSPN overexpression results in upregulation of key signaling molecules associated with regulation of cytoskeleton organization and mechanotransduction, including Yap1, Sox9, Rho, RAC, and Wnt.ConclusionsOur findings indicate that SSPN overexpression rescues dystrophin deficiency partially through mechanotransduction signaling cascades mediated through components of the ECM and the cortical cytoskeleton.

【 授权许可】

CC BY   
© The Author(s) 2022

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