Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T

AMB Express

Original Article

Lingmin Jiang¹  Cha Young Kim¹  Jiyoung Lee¹  Jiyoon Seo¹  Doeun Jeon¹  Ju Huck Lee¹  Yuxin Peng¹  Pyoung Il Kim²  Soon Ju Park³  Chul Hong Kim⁴ 

[1] Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, 56212, Jeongeup, Republic of Korea;Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), 56212, Jeongeup, Republic of Korea;Division of Applied Life Science, Gyeongsang National University, 52828, Jinju, Republic of Korea;Hyungnong Co., Ltd., 57512, Gokseong, Republic of Korea;

关键词: Endophyte;    Saccharibacillus brassicae;    Plant growth-promotion;    Whole-genome;    antiSMASH;   

DOI  :  10.1186/s13568-023-01514-1

 received in 2022-07-21, accepted in 2023-01-09,  发布年份 2023

来源: Springer

【 摘 要 】

Endophytes can facilitate the improvement of plant growth and health in agriculturally important crops, yet their genomes and secondary metabolites remain largely unexplored. We previously isolated Saccharibacillus brassicae strain ATSA2T from surface-sterilized seeds of kimchi cabbage and represented a novel species of the genus Saccharibacillus. In this study, we evaluated the plant growth-promoting (PGP) effect of strain ATSA2T in kimchi cabbage, bok choy, and pepper plants grown in soils. We found a significant effect on the shoot and root biomass, and chlorophyll contents following strain ATSA2T treatment. Strain ATSA2T displayed PGP traits such as indole acetic acid (IAA, 62.9 μg/mL) and siderophore production, and phosphate solubilization activity. Furthermore, genome analysis of this strain suggested the presence of gene clusters involved in iron acquisition (fhuABD, afuABC, fbpABC, and fepCDG) and phosphate solubilization (pstABCHS, phoABHLU, and phnCDEP) and other phytohormone biosynthesis genes, including indole-3-acetic acid (trpABCDEFG), in the genome. Interestingly, the secondary metabolites cerecidin, carotenoid, siderophore (staphylobactin), and bacillaene underlying plant growth promotion were found in the whole genome via antiSMASH analysis. Overall, physiological testing and genome analysis data provide comprehensive insights into plant growth-promoting mechanisms, suggesting the relevance of strain ATSA2T in agricultural biotechnology.

【 授权许可】

CC BY   
© The Author(s) 2023

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