Changes in type 2 innate lymphoid cells and serum cytokines in sublingual immunotherapy in pediatric patients with allergic rhinitis

BMC Pediatrics

Research Article

Yang Shen¹  Houyong Kang¹  Suling Hong¹  Xia Ke¹  Xiaoqiang Wang¹ 

[1] Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, 400016, Chongqing, People’s Republic of China;

关键词: Sublingual immunotherapy;    Allergic rhinitis;    Type 2 innate lymphocytes;    Pediatric patients;   

DOI  :  10.1186/s12887-022-03788-z

 received in 2021-11-30, accepted in 2022-12-01,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundType 2 innate lymphoid cells (ILC2) are upregulated in childhood allergic rhinitis (AR) and are associated with AR severity. This study aimed to investigate changes in the ILC2 milieu in pediatric patients with AR after sublingual immunotherapy (SLIT).MethodsForty- pediatric patients with AR received house dust mite (HDM) allergen extract for SLIT group and thirty pediatric patients received placebo in the study, respectively. The levels of ILC2, ILC2-related cytokines (IL-5/IL-13) and their transcription factors (GATA binding protein 3, retinoic acid-related orphan receptor α) in the circulation were assessed after 1- and 2-year SLIT. Moreover, peripheral blood mononuclear cells (PBMCs) in patients were prepared and stimulated by recombinant thymic stromal lymphopoietin, IL-25, and IL-33 after 2-year SLIT. Subsequently, the levels of ILC2, IL-5, and IL-13 were tested.ResultsThe frequency of ILC2 and the levels of their transcription factors in the circulation were significantly decreased after SLIT in the SLIT group. The levels of ILC2-related cytokines in the SLIT group showed the same trend. The frequency of ILC2 was positively correlated with transcription factors and cytokines after SLIT. SLIT was observed to reduce the ability of HDM sensitization to generate the ILC2 milieu in PBMCs.ConclusionsChanges in the ILC2 milieu may be correlated with the curative effect and immune regulation function of SLIT. Our results suggested that the regulatory effect on ILC2 is part of the therapeutic mechanism of SLIT.

【 授权许可】

CC BY   
© The Author(s) 2023

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Fig. 1

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Fig. 2

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