Selenium and/or vitamin E upregulate the antioxidant gene expression and parameters in broilers

BMC Veterinary Research

Research

Sabreen E. Fadl¹  Shabaan A. Hemeda²  Aaser M. Abdelazim³  Muhanad Alhujaily⁴  Rasha A. Al Wakeel⁵  Fatma Elgendey⁶  Olla A. Khalifa⁶  Aya Mohamed Elshwash⁶ 

[1] Biochemistry Department, Faculty of Veterinary Medicine, Matrouh University, 51744, Matrouh, Egypt;Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, 21526, Alexandria, Egypt;Department of Basic Medical Sciences, College of Aapplied Medical Sciences, University of Bisha, 61922, Bisha, Saudi Arabia;Department of Medical Laboratory Sciences, College of Applied Medical Sciences, University of Bisha, 61922, Bisha, Saudi Arabia;Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516, Kafrelsheikh, Egypt;Genetics and Genetic Engineering, Department of Animal Wealth Development, Faculty of Veterinary Medicine, Benha University, 13736, Toukh, Egypt;

关键词: Antioxidants;    Broilers;    Copper and iron;    Gene expression;    Selenium;    Vitamin E;   

DOI  :  10.1186/s12917-022-03411-4

 received in 2022-03-04, accepted in 2022-08-04,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIn contrast to free radicals, the first line of protection is assumed to be vitamin E and selenium. The present protocol was designed to assess the roles of vitamin E and/or a selenium-rich diet that affected the blood iron and copper concentrations, liver tissue antioxidant and lipid peroxidation, and gene expression linked to antioxidants in the liver tissue of broilers. The young birds were classified according to the dietary supplement into four groups; control, vitamin E (100 mg Vitamin/kg diet), selenium (0.3 mg sodium selenite/kg diet), and vitamin E pulse selenium (100 mg vitamin/kg diet with 0.3 mg sodium selenite/kg diet) group.ResultsThe results of this experiment suggested that the addition of vitamin E with selenium in the broiler diet significantly increased (P ≤ 0.05) serum iron when compared with the other groups and serum copper when compared with the vitamin E group. Moreover, the supplements (vitamin E or vitamin E with selenium) positively affected the enzymatic activity of the antioxidant-related enzymes with decreased malondialdehyde (MDA),which represents lipid peroxidation in broiler liver tissue. Moreover, the two supplements significantly upregulated genes expression related to antioxidants.ConclusionTherefore, vitamin E and/or selenium can not only act as exogenous antioxidants to prevent oxidative damage by scavenging free radicals and superoxide, but also act as gene regulators, regulating the expression of endogenous antioxidant enzymes.

【 授权许可】

CC BY   
© The Author(s) 2022. corrected publication 2023

【 图 表 】

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