QTL mapping and candidate gene analysis for yield and grain weight/size in Tartary buckwheat

BMC Plant Biology

Research

Ruiyuan Li¹  Taoxiong Shi²  Ran Zheng²  Hongyou Li²  Zhengfeng Chen²  Juan Huang²  Chenggang Liang²  Qingfu Chen²  Jiao Deng² 

[1]Key Laboratory of Information and Computing Science of Guizhou Province, Guizhou Normal University, 550001, Guiyang, Guizhou, China

[2]Research Center of Buckwheat Industry Technology, Guizhou Normal University, 550001, Guiyang, Guizhou, China

关键词: Tartary buckwheat;    Yield;    Grain weight/size;    QTL;    SNP/InDel variation;    Candidate gene;   

DOI  :  10.1186/s12870-022-04004-x

 received in 2022-10-05, accepted in 2022-12-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundGrain weight/size influences not only grain yield (GY) but also nutritional and appearance quality and consumer preference in Tartary buckwheat. The identification of quantitative trait loci (QTLs)/genes for grain weight/size is an important objective of Tartary buckwheat genetic research and breeding programs.ResultsHerein, we mapped the QTLs for GY, 1000-grain weight (TGW), grain length (GL), grain width (GW) and grain length–width ratio (L/W) in four environments using 221 recombinant inbred lines (XJ-RILs) derived from a cross of 'Xiaomiqiao × Jinqiaomai 2'. In total, 32 QTLs, including 7 for GY, 5 for TGW, 6 for GL, 11 for GW and 3 for L/W, were detected and distributed in 24 genomic regions. Two QTL clusters, qClu-1-3 and qClu-1-5, located on chromosome Ft1, were revealed to harbour 7 stable major QTLs for GY (qGY1.2), TGW (qTGW1.2), GL (qGL1.1 and qGL1.4), GW (qGW1.7 and qGW1.10) and L/W (qL/W1.2) repeatedly detected in three and above environments. A total of 59 homologues of 27 known plant grain weight/size genes were found within the physical intervals of qClu-1-3 and qClu-1-5. Six homologues, FtBRI1, FtAGB1, FtTGW6, FtMADS1, FtMKK4 and FtANT, were identified with both non-synonymous SNP/InDel variations and significantly differential expression levels between the two parents, which may play important roles in Tatary buckwheat grain weight/size control and were chosen as core candidate genes for further investigation.ConclusionsTwo stable major QTL clusters related to grain weight/size and six potential key candidate genes were identified by homology comparison, SNP/InDel variations and qRT‒qPCR analysis between the two parents. Our research provides valuable information for improving grain weight/size and yield in Tartary buckwheat breeding.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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