FGF23 promotes proliferation, migration and invasion by regulating miR-340-5p in osteosarcoma

Journal of Orthopaedic Surgery and Research

Research Article

Lun Fang¹  Beilei Yu¹  Zhongzhe Li¹  Lu Zhou² 

[1] Institute of Sports Medicine, Shandong First Medical University and Shandong Academy Medical Sciences, 619 Changcheng Road, 271016, Taian, Shandong, People’s Republic of China;Institute of Sports Medicine, Shandong First Medical University and Shandong Academy Medical Sciences, 619 Changcheng Road, 271016, Taian, Shandong, People’s Republic of China;Clinical Center for Sports Medicine and Rehabilitation, The Affiliated Hospital of Shandong First Medical University, 706 Taishan Great Street, 271000, Taian, Shandong, People’s Republic of China;

关键词: Osteosarcoma;    FGF23;    miR-340-5p;    Proliferation;    Migration;    Invasion;   

DOI  :  10.1186/s13018-022-03483-w

 received in 2022-11-15, accepted in 2022-12-29,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIncreasing evidences have been indicated that FGF23 is associated with the biological behavior of malignant tumors, but its role in osteosarcoma and the specific mechanism need to be elucidated. The purpose of this study is to investigate the effects of FGF23 on the proliferation, migration and invasion of osteosarcoma cells, and the possible molecular mechanisms.MethodsWestern blot was used to detect differences in FGF23 expression in osteosarcoma cells MG-63 and U2-OS and osteoblasts hFOB1.19. FGF23-overexpressing adenoviruses and FGF-silencing plasmids were transfected into osteosarcoma cells, and transfection efficiency was verified using Western blot. MTT and colony formation assays were performed to detect osteosarcoma cell proliferation. Cell cycle was measured by flow cytometry. Scratch assay, holographic imaging cell analyzer Holomonitor ® M4 and transwell were applied to detect cell migration and invasion. Dual-luciferase reporter assay was performed to validate the interaction between FGF23 and miR-340-5p. Changes in miR-340-5p mRNA levels were measured by QRT-PCR.ResultsFGF23 is highly expressed in osteosarcoma cells compared to hFOB1.19. Overexpression of FGF23 significantly promoted the proliferation, migration and invasion of MG-63 and U2-OS cells. MiR-340-5p is a target of FGF23. Transfection of miR-340-5p mimics reversed the promoting effects of FGF23 on proliferation, migration and invasion of MG-63 and U2-OS cells.ConclusionFGF23 promotes osteosarcoma cell proliferation, migration and invasion by targeting miR-340-5p gene expression.

【 授权许可】

CC BY   
© The Author(s) 2023

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