LncRNA–mRNA expression profile and functional network of vascular dysfunction in septic rats

European Journal of Medical Research

Research

Zhu-Jun Shen¹  Ye-Chen Han¹  Yi-Ning Wang¹  Hong-Zhi Xie¹  Ruo-Lan Xiang² 

[1]Department of Cardiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No.1 Shuaifuyuan, Dongcheng District, 100730, Beijing, China

[2]Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, 100191, Beijing, China

关键词: Sepsis;    Aorta;    Long non‑coding RNA;    Profile;   

DOI  :  10.1186/s40001-022-00961-z

 received in 2022-06-08, accepted in 2022-12-19,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundWe used microarrays to analyse the changes in long non-coding RNAs (lncRNAs) and mRNAs in aorta tissue in model rats with lipopolysaccharide-induced sepsis and determined the lncRNA–mRNA and lncRNA–miRNA–mRNA functional networks.MethodsWistar rats were intraperitoneally injected with lipopolysaccharide, and the lncRNA and mRNA expression profiles in the aorta were evaluated using microarrays. The functions of the differentially expressed mRNAs were analysed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. We then constructed coding/non-coding co-expression and competing endogenous RNA networks to study the mechanisms related to sepsis in rats.ResultsWe identified 503 differentially expressed lncRNAs and 2479 differentially expressed mRNAs in the model rats with lipopolysaccharide-induced sepsis. Mitochondrial fission process 1 (MTFP1) was the most significantly down-regulated mRNA. Bioinformatics analysis showed that the significantly down-regulated mRNAs in the sepsis models were in pathways related to mitochondrial structure, function, and energy metabolism. Coding/non-coding co-expression and competing endogenous RNA analyses were conducted using 12 validated lncRNAs in combination with all mRNAs. The coding/non-coding co-expression analysis showed that the 12 validated lncRNAs were mainly regulatory factors for abnormal energy metabolism, including mitochondrial structure damage and aberrant mitochondrial dynamics. The competing endogenous RNA analysis revealed that the potential functions of these 12 lncRNAs might be related to the inflammatory response.ConclusionWe determined the differentially expressed lncRNAs and mRNAs in the aorta of septic rats using microarrays. Further studies on these lncRNAs will help elucidate the mechanism of sepsis at the genetic level and may identify potential therapeutic targets.

【 授权许可】

CC BY   
© The Author(s) 2023

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