Development of a methodology for the characterisation and assessment of biodeteriogens on archaeological surfaces by use of a portable LED-induced fluorescence instrument

Heritage Science

Research

A. Giakoumaki¹  A. Philippidis¹  P. Pouli¹  P. Siozos¹  D. Anglos²  I. Pyrri³ 

[1] Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (IESL-FORTH), 100 N. Plastira St., 70013, Heraklion, Crete, Greece;Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (IESL-FORTH), 100 N. Plastira St., 70013, Heraklion, Crete, Greece;Department of Chemistry, University of Crete, 71003, Heraklion, Crete, Greece;Section of Ecology and Systematics, Department of Biology, School of Science, National and Kapodistrian University of Athens, Panepistimioupoli, 15784, Athens, Greece;

关键词: Stone monuments;    Biodeterioration;    LED-induced fluorescence;    Portable instrumentation;   

DOI  :  10.1186/s40494-022-00827-x

 received in 2022-06-02, accepted in 2022-11-09,  发布年份 2022

来源: Springer

【 摘 要 】

The present study focuses on the development of a fast, non-invasive methodology, appropriate for the detection and characterization of biodeterioration present on the surface of archaeological/historical stone objects and monuments, by exploiting the characteristic fluorescence emission of biological deposits. Fluorescence spectra were collected by use of a portable LED (Light Emitting Diode)-Induced Fluorescence (LED-IF) instrument. Three limestone fragments and one mortar fragment, from different monuments in Greece, presenting various types of biodeterioration on their surface, have been investigated in the laboratory. First, fluorescence emission spectra were acquired with a benchtop laboratory spectrofluorometer in order to select the optimum excitation wavelengths for the fluorophores present in the biological crust. An evaluation of the portable LED-IF instrument was conducted by assessing the performance of its optical components and different LED excitation sources, while an investigation of several experimental parameters on the fluorescence signal was also performed. Furthermore, issues related to the efficiency of detection and identification of biological growth have been studied, such as the effect of sample surface wetting on the fluorescence signal. The results of the present study demonstrate that the LED-IF instrument can be used for a fast and reliable assessment of the presence of biodeterioration on monuments.

【 授权许可】

CC BY   
© The Author(s) 2022

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