Silencing of StRIK in potato suggests a role in periderm related to RNA processing and stress

BMC Plant Biology

Research Article

Sebastian Y. Müller¹  Krystyna A. Kelly¹  Marçal Soler²  Xènia Torrent²  Pau Boher²  Sandra Fernández-Piñán²  Mercè Figueras²  Olga Serra² 

[1] Department of Plant Sciences, University of Cambridge, Downing Street, CB2 3EA, Cambridge, UK;Laboratori del Suro, Biology Department, Universitat de Girona, Campus Montilivi, E-17071, Girona, Catalonia, Spain;

关键词: RS2-INTERACTING KH PROTEIN;    Potato tuber periderm;    Flowering;    RNA regulation;    KH-domain RNA-binding protein;   

DOI  :  10.1186/s12870-021-03141-z

 received in 2021-01-21, accepted in 2021-07-06,  发布年份 2021

来源: Springer

【 摘 要 】

BackgroundThe periderm is a protective barrier crucial for land plant survival, but little is known about genetic factors involved in its development and regulation. Using a transcriptomic approach in the cork oak (Q. suber) periderm, we previously identified an RS2-INTERACTING KH PROTEIN (RIK) homologue of unknown function containing a K homology (KH)-domain RNA-binding protein, as a regulatory candidate gene in the periderm.ResultsTo gain insight into the function of RIK in the periderm, potato (S. tuberosum) tuber periderm was used as a model: the full-length coding sequence of RIK, hereafter referred to as StRIK, was isolated, the transcript profile analyzed and gene silencing in potato performed to analyze the silencing effects on periderm anatomy and transcriptome. The StRIK transcript accumulated in all vegetative tissues studied, including periderm and other suberized tissues such as root and also in wounded tissues. Downregulation of StRIK in potato by RNA interference (StRIK-RNAi) did not show any obvious effects on tuber periderm anatomy but, unlike Wild type, transgenic plants flowered. Global transcript profiling of the StRIK-RNAi periderm did show altered expression of genes associated with RNA metabolism, stress and signaling, mirroring the biological processes found enriched within the in silico co-expression network of the Arabidopsis orthologue.ConclusionsThe ubiquitous expression of StRIK transcript, the flower associated phenotype and the differential expression of StRIK-RNAi periderm point out to a general regulatory role of StRIK in diverse plant developmental processes. The transcriptome analysis suggests that StRIK might play roles in RNA maturation and stress response in the periderm.

【 授权许可】

CC BY   
© The Author(s) 2021. corrected publication 2022

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