Genome-wide association study reveals the genetic determinism of serum biochemical indicators in ducks

BMC Genomics

Research Article

Wenlei Fan¹  Hehe Tang²  Zhengkui Zhou²  Zhanbao Guo²  Shuisheng Hou²  He Zhang²  Daxin Yu²  Dapeng Liu²  Wei Huang²  Zhen Wang² 

[1] College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, PR China;Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, PR China;

关键词: Serum;    Biochemical indicators;    GWAS;    Duck;   

DOI  :  10.1186/s12864-022-09080-9

 received in 2022-08-20, accepted in 2022-12-12,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe serum is rich in nutrients and plays an essential role in electrolyte and acid–base balance, maintaining cellular homeostasis. In addition, serum parameters have been commonly used as essential biomarkers for clinical diagnosis. However, little is known about the genetic mechanism of the serum parameters in ducks.ResultsThis study measured 18 serum parameters in 320 samples of the F2 segregating population generated by Mallard × Pekin duck. The phenotypic correlations showed a high correlation between LDH, HBDH, AST, and ALT (0.59–0.99), and higher coefficients were also observed among TP, ALB, HDL-C, and CHO (0.46–0.87). And then, we performed the GWAS to reveal the genetic basis of the 18 serum biochemical parameters in ducks. Fourteen candidate protein-coding genes were identified with enzyme traits (AST, ALP, LDH, HBDH), and 3 protein-coding genes were associated with metabolism and protein-related serum parameters (UA, TG). Moreover, the expression levels of the above candidate protein-coding genes in different stages of breast muscle and different tissues were analyzed. Furthermore, the genes located within the high-LD region (r2 > 0.4 and − log10(P) < 4) neighboring the significant locus also remained. Finally, 86 putative protein-coding genes were used for GO and KEGG enrichment analysis, the enzyme-linked receptor protein signaling pathway and ErbB signaling pathway deserve further focus.ConclusionsThe obtained results can contribute to new insights into blood metabolism and provide new genetic biomarkers for application in duck breeding programs.

【 授权许可】

CC BY   
© The Author(s) 2022

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