Genome-wide identification of BBX gene family and their expression patterns under salt stress in soybean

BMC Genomics

Research

Lulu Zhai¹  Shaomin Bian¹  Guohua Bao¹  Tianran Shi¹  Xuyan Li¹  Binghui Shan¹ 

[1] College of Plant Science, Jilin University, Changchun, China;

关键词: Soybean;    BBX family;    Phylogenetic evolution;    Salt stress;    Gene expression;    Prediction of protein interaction;    Binding sites of transcription factor;   

DOI  :  10.1186/s12864-022-09068-5

 received in 2022-08-30, accepted in 2022-12-05,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundBBX genes are key players in the regulation of various developmental processes and stress responses, which have been identified and functionally characterized in many plant species. However, our understanding of BBX family was greatly limited in soybean.ResultsIn this study, 59 BBX genes were identified and characterized in soybean, which can be phylogenetically classified into 5 groups. GmBBXs showed diverse gene structures and motif compositions among the groups and similar within each group. Noticeably, synteny analysis suggested that segmental duplication contributed to the expansion of GmBBX family. Moreover, our RNA-Seq data indicated that 59 GmBBXs showed different transcript profiling under salt stress, and qRT-PCR analysis confirmed their expression patterns. Among them, 22 GmBBXs were transcriptionally altered with more than two-fold changes by salt stress, supporting that GmBBXs play important roles in soybean tolerance to salt stress. Additionally, Computational assay suggested that GmBBXs might potentially interact with GmGI3, GmTOE1b, GmCOP1, GmCHI and GmCRY, while eight types of transcription factors showed potentials to bind the promoter regions of GmBBX genes.ConclusionsFifty-nine BBX genes were identified and characterized in soybean, and their expression patterns under salt stress and computational assays suggested their functional roles in response to salt stress. These findings will contribute to future research in regard to functions and regulatory mechanisms of soybean BBX genes in response to salt stress.

【 授权许可】

CC BY   
© The Author(s) 2022

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