Countrywide insecticide resistance monitoring and first report of the presence of the L1014S knock down resistance in Niger, West Africa

Malaria Journal

Research

Rabiou Labbo¹  Noura Maman Salé¹  Halima Naroua Zamaka¹  Ibrahim Issa Arzika¹  Izamné Mahamadou¹  Souleymane Mahaman Iro¹  Amadou Soumana¹  Boubacar Mahamadou¹  Saadou Kadri¹  Wilfried Hounkanrin¹  Ibrahim Maman Laminou¹  Jenny Carlson²  Fatoumata Abdoulaye Daouda³  Abdoulaye Daouda³  Maman Sani Zaman-Allah³  Samira Gouro³  Boubé Hamani³  Sabiti Idrissa³  Hadiza Jackou³  Hadiza Soumaila⁴  Joseph Chabi⁵  Cecilia Flatley⁵  Taiwo Samson Awolola⁶  Ellen Dotson⁶  Eric Coulibaly⁷  Zilahatou Bahari-Tohon⁷  Els Mathieu⁸ 

[1] Centre de Recherche Médicale et Sanitaire, Niamey, Niger;Entomology Branch, U.S. President’s Malaria Initiative, Atlanta, GA, USA;National Malaria Control Programme, Niamey, Niger;PMI VectorLink Project, Niamey, Niger;PMI VectorLink Project, Washington, DC, USA;U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA;U.S. President’s Malaria Initiative, USAID, Niamey, Niger;U.S. President’s Malaria Initiative, USAID, Niamey, Niger;U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA;

关键词: Insecticide resistance;    Resistance mechanisms;    New generation ITNs;    Malaria;    Niger;   

DOI  :  10.1186/s12936-022-04410-4

 received in 2022-06-11, accepted in 2022-12-09,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundMass distribution of insecticide-treated nets (ITNs) is the principal malaria vector control strategy adopted by Niger. To better inform on the most appropriate ITN to distribute, the National Malaria Control Programme (NMCP) of Niger and its partners, conducted insecticide resistance monitoring in selected sites across the country.MethodsThe susceptibility of Anopheles gambiae sensu lato (s.l.) to chlorfenapyr and pyrethroid insecticides was investigated in a total of sixteen sites in 2019 and 2020, using 2–5-day-old adults reared from wild collected larvae per site. The susceptibility status, pyrethroid resistance intensity at 5 and 10 times the diagnostic concentrations, and piperonyl butoxide (PBO) synergism with diagnostic concentrations of deltamethrin, permethrin and alpha-cypermethrin were assessed using WHO bioassays. Two doses (100 and 200 µg/bottle) of chlorfenapyr were tested using the CDC bottle assay method. Species composition and allele frequencies for knock-down resistance (kdr-L1014F and L1014S) and acetylcholinesterase (ace-1 G119S) mutations were further characterized using polymerase chain reaction (PCR).ResultsHigh resistance intensity to all pyrethroids tested was observed in all sites except for alpha-cypermethrin in Gaya and Tessaoua and permethrin in Gaya in 2019 recording moderate resistance intensity. Similarly, Balleyara, Keita and Tillabery yielded moderate resistance intensity for alpha-cypermethrin and deltamethrin, and Niamey V low resistance intensity against deltamethrin and permethrin in 2020. Pre-exposure to PBO substantially increased susceptibility with average increases in mortality between 0 and 70% for tested pyrethroids. Susceptibility to chlorfenapyr (100 µg/bottle) was recorded in all sites except in Tessaoua and Magaria where susceptibility was recorded at the dose of 200 µg/bottle. Anopheles coluzzii was the predominant malaria vector species in most of the sites followed by An. gambiae sensu stricto (s.s.) and Anopheles arabiensis. The kdr-L1014S allele, investigated for the first time, was detected in the country. Both kdr-L1014F (frequencies [0.46–0.81]) and L1014S (frequencies [0.41–0.87]) were present in all sites while the ace-1 G119S was between 0.08 and 0.20.ConclusionThe data collected will guide the NMCP in making evidence-based decisions to better adapt vector control strategies and insecticide resistance management in Niger, starting with mass distribution of new generation ITNs such as interceptor G2 and PBO ITNs.

【 授权许可】

CC BY   
© The Author(s) 2022

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