In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae

Porcine Health Management

Research

Manuel Gómez-García¹  Clara Vega¹  Héctor Puente¹  Lucía Pérez-Pérez¹  Héctor Argüello²  Ana Carvajal²  Pedro Rubio²  Ignacio Fernández-Usón³ 

[1] Department of Animal Health, Faculty of Veterinary Medicine, Universidad de León, Campus de Vegazana S/N, 24007, León, Spain;Department of Animal Health, Faculty of Veterinary Medicine, Universidad de León, Campus de Vegazana S/N, 24007, León, Spain;INDEGSAL, Universidad de León, Campus de Vegazana S/N, 24007, León, Spain;Fatro Ibérica S.L., C/ Constitución 1 PB3, Sant Just Desvern, 08960, Barcelona, Spain;

关键词: Spirochaetes;    Antibiotic;    Aminoglycoside;    Swine dysentery;    Antimicrobial susceptibility testing;    Resistance;   

DOI  :  10.1186/s40813-022-00291-w

 received in 2022-07-01, accepted in 2022-10-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe treatment of swine dysentery (SD) has become constrained in recent years due to the limited availability of effective drugs combined with a rise in antimicrobial resistance. Gentamicin, an aminoglycoside antibiotic, is authorised for the control of this disease in several European countries but has not been extensively used so far. In this study, the in vitro susceptibility of 56 Brachyspira hyodysenteriae field isolates was evaluated against gentamicin using a broth microdilution test. The molecular basis of decreased susceptibility to gentamicin was also investigated by sequencing the 16S rRNA gene and phylogenetic relatedness by multiple-locus variable number tandem-repeat analysis (MLVA).ResultsMost B. hyodysenteriae isolates presented low minimum inhibitory concentration (MIC) values to gentamicin, with a mode of 2 µg/mL, a median or MIC50 of 4 µg/mL and percentile 90 or MIC90 of 16 µg/mL. The distribution of these values over the period studied (2011–2019) did not show a tendency towards the development of resistance to gentamicin. Differences in susceptibility among isolates could be explained by two point-mutations in the 16S rRNA gene, C990T and A1185G, which were only present in isolates with high MICs. These isolates were typed in three different MLVA clusters. Analyses of co-resistance between gentamicin and antimicrobials commonly used for the treatment of SD revealed that resistance to tiamulin and valnemulin was associated with low MICs for gentamicin.ConclusionsThe results provide an accurate characterisation of antimicrobial sensitivity to gentamicin and possible mechanisms of resistance in Spanish B. hyodysenteriae isolates. These findings allow us to propose gentamicin as an alternative in the antibiotic management of SD, particularly in outbreaks caused by pleuromutilin resistant isolates.

【 授权许可】

CC BY   
© The Author(s) 2022

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