BMC Complementary Medicine and Therapies | |
In vitro and in silico analysis of ‘Taikong blue’ lavender essential oil in LPS-induced HaCaT cells and RAW264.7 murine macrophages | |
Research | |
Jie Xiang1  Mengya Wei1  Junsong Xiao1  Rifat Nowshin Raka1  Hua Wu1  Suzhen Yang2  Tingting Han2  Fei Liu2  Fengjiao Guo2  | |
[1] Beijing Technology and Business University, 100048, Beijing, China;Shandong Freda Biotech Co., Ltd, 250101, Ji’nan, Shandong, China;Xinjiang Eprhan Spices Co., Ltd, 835213, Cocodala, Xinjiang, China; | |
关键词: Lavender essential oil; Skin; Anti-inflammatory; Antioxidative; Macrophages; | |
DOI : 10.1186/s12906-022-03800-0 | |
received in 2022-08-04, accepted in 2022-11-17, 发布年份 2022 | |
来源: Springer | |
【 摘 要 】
Background‘Taikong blue’ lavender, a space-bred cultivar of Lavandula angustifolia, is one of the main lavender essential oil production crops in Xinjiang Province, China. Several cases of local usage indicated that ‘Taikong blue’ lavender essential oil (TLEO) had excellent anti-inflammatory and antioxidant properties for skin problems. However, to date, substantial data on these functions are lacking. In this study, we aimed to investigate the composition and bioactivities of TLEO and the potential underlying mechanisms through LPS-induced inflammatory models of HaCaT and RAW264.7 cells.MethodsThe composition of TLEO was determined by GC‒MS. To study the anti-inflammatory and antioxidative properties of TLEO, we induced HaCaT and RAW264.7 cells by LPS. TLEO (0.001%-0.1%, v/v) was used to treat inflamed cells with dexamethasone (DEX, 10 μg/mL) as the standard drug. A variety of tests were carried out, including biochemical assays, ELISA, RT‒PCR, and western blotting. Docking of components was performed to predict potential ligands.ResultsThe GC‒MS analysis revealed that 53 compounds (> 0.01%) represented 99.76% of the TLEO, and the majority of them were esters. TLEO not only reduced the levels of oxidative stress indicators (NO, ROS, MDA, and iNOS at the mRNA and protein levels) but also protected the SOD and CAT activities. According to the RT‒PCR, ELISA, and Western blot results, TLEO decreased inflammation by inhibiting the expression of TNF-α, IL-1β, IL-6, and key proteins (IκBα, NF-кB p65, p50, JNK, and p38 MAPK) in MAPK-NF-кB signaling. Molecular docking results showed that all of the components (> 1% in TLEO) were potent candidate ligands for further research.ConclusionThe theoretical evidence for TLEO in this study supported its use in skin care as a functional ingredient for cosmetics and pharmaceutics.Graphical Abstract
【 授权许可】
CC BY
© The Author(s) 2022
【 预 览 】
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RO202305067963558ZK.pdf | 6023KB | download | |
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