NCAPG promotes the oncogenesis and progression of non-small cell lung cancer cells through upregulating LGALS1 expression

Molecular Cancer

Research

Hong Zhang¹  Yan Yan²  Yushi Li²  Cuiling Zhou²  Gang Che²  Christophe Nicot³  Huanhuan Sun⁴  Haiqing Ma⁵ 

[1] Department of Oncology, Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China;Department of Oncology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China;Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, 3901 Rainbow Boulevard, 66160, Kansas City, KS, USA;Medical Research Center, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Rd, 510080, Guangzhou, Guangdong, China;Department of Oncology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China;Medical Research Center, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, 106 Zhongshan Er Rd, 510080, Guangzhou, Guangdong, China;Department of Oncology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China;Department of Oncology, Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China;

关键词: NSCLC;    NCAPG;    LGALS1;    Oncogenesis;    Progression;   

DOI  :  10.1186/s12943-022-01533-9

 received in 2021-11-03, accepted in 2022-02-08,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundNumerous common oncogenic driver events have been confirmed in non-small cell lung cancer (NSCLC). Although targeted therapy has revolutionized NSCLC treatment, some patients still do not respond. NCAPG, also known as non-SMC condensin I complex subunit G, was positively associated with proliferation and migration in several tumor types.MethodsWe used transcriptional sequencing and TCGA database analysis to identify NCAPG as a new therapeutic target for NSCLC. The oncogenic roles of NCAPG in NSCLC tumor growth and metastasis were detected in vitro and in vivo. Ncapg+/+ or Ncapg+/− mice with urethane treatment were analyzed for oncogenesis of NSCLC.ResultsWe investigated NCAPG as a new oncogenic driver which promoted NSCLC tumorigenesis and progression. We used transcriptome sequencing and the Cancer Genome Atlas (TCGA) database analysis to screen and found that NCAPG was negatively correlated with NSCLC survival. Using immunohistochemistry, we demonstrated that NCAPG overexpression was an independent risk factor for NSCLC survival. Functionally, NCAPG knockdown inhibited proliferation, migration, and invasion of NSCLC cells in vitro and in vivo. We exposed wildtype or Ncapg+/− mice to urethane and discovered that urethane-induced lung tumors were reduced in Ncapg+/− mice. Mechanistically, the function of NCAPG in promoting initiation and progression of NSCLC was closely related to LGALS1, which was also upregulated in NSCLC and might interact directly with NCAPG.ConclusionsThis study indicates that NCAPG is one of the essential factors for NSCLC oncogenesis and progression, providing a new target for prognosis prediction and treatment of NSCLC.

【 授权许可】

CC BY   
© The Author(s) 2022

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