A biostatistical approach for augmenting rare bianthraquinone antibiotic production by Streptomyces sp. RA-WS2 using Taguchi design

AMB Express

Original Article

Ravi Singh Manhas¹  Asha Chaubey¹  Amit Kumar² 

[1] Fermentation & Microbial Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, 180001, Jammu, India;Academy of Scientific and Innovative Research, CSIR-Human Resource Development Centre, Campus Ghaziabad, 201002, Ghaziabad, India;Quality Management & Instrumentation Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, 180001, Jammu, India;Academy of Scientific and Innovative Research, CSIR-Human Resource Development Centre, Campus Ghaziabad, 201002, Ghaziabad, India;

关键词: Streptomyces;    Fermentation;    9,9’-bianthraquinone;    Setomimycin;    Optimization Taguchi design;   

DOI  :  10.1186/s13568-022-01497-5

 received in 2022-09-08, accepted in 2022-11-24,  发布年份 2022

来源: Springer

【 摘 要 】

Consistent production of bioactives from microbial sources remains a big challenge for fermentation based bio-processes. Setomimycin, a rare 9,9'-bianthrylanthracene antibiotic reported to be active against Gram positive bacteria i.e. Staphyloccocus aureus, Bacillus subtilis, Bacillus cereus, and Mycobacterium smegmatis, including mycobacteria is one of the least exploited antibiotic. Present work aims to enhance and maximize setomimycin production using One Factor at a Time (OFAT) approach, followed by Taguchi L9 orthogonal array (OA) design in 30L fermenter. Four most influential parameters, namely carbon source, nitrogen source, air and agitation were selected for optimization studies. The optimized production medium supplemented with 150 g/L glycerol and 7.5 g/L soyabean meal with an agitation rate of 100 RPM and air flow rate of 20 LPM (Liters Per Minute) resulted in 675 mg/L setomimycin production within 96–108 h of fermentation as compared to the initial production i.e. 40 mg/L. Thus, an overall enhancement of 16.8 folds was achieved in setomimycin production after optimization in 30L fermenter.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2022

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