Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses

Journal of Ovarian Research

Research

Raymond Simonot¹  Nicolas Lieury¹  Déborah Bourc’his²  Adrien Guilloteau³  Bastien Ducreux⁴  Magali Guilleman⁵  Céline Bruno⁵  Julie Barberet⁵  Patricia Fauque⁶ 

[1] CHU Dijon Bourgogne, Laboratoire de Biologie de la Reproduction – CECOS, 14 rue Gaffarel, F-21000, Dijon, France;Institut Curie, PSL University, CNRS, INSERM, 26 rue d’Ulm, F-75248, Paris, France;USMR, Dijon Bourgogne University Hospital, F-21000, Dijon, France;Université Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231, 2 Rue Angélique Ducoudray, F-21000, Dijon, France;Université Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231, 2 Rue Angélique Ducoudray, F-21000, Dijon, France;CHU Dijon Bourgogne, Laboratoire de Biologie de la Reproduction – CECOS, 14 rue Gaffarel, F-21000, Dijon, France;Université Bourgogne Franche-Comté - Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231, 2 Rue Angélique Ducoudray, F-21000, Dijon, France;CHU Dijon Bourgogne, Laboratoire de Biologie de la Reproduction – CECOS, 14 rue Gaffarel, F-21000, Dijon, France;Laboratoire de Biologie de la Reproduction, CHU Dijon, BP 77908, 14, rue Gaffarel, 21079, Dijon Cedex, France;

关键词: Assisted reproductive technology;    Cryopreservation;    Oocyte;    Single-cell RNA-seq;    Vitrification;   

DOI  :  10.1186/s13048-022-01064-3

 received in 2022-07-07, accepted in 2022-11-21,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIndications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology.ResultsPost-warming survival rate, oocyte surfaces and resistance to empty micro-injection were comparable between semi-automated and manual vitrification groups. Both oocyte vitrification techniques showed limited differences in the resulting transcriptomic profile of sibling oocytes since only 5 differentially expressed genes were identified. Additionally, there was no difference in median transcript integrity number or percentage of mitochondrial DNA between the two groups. However, a total of 108 genes were differentially expressed between fresh and vitrified oocytes (FDR < 0.05) and showed over-represented of genes related to important cellular process.ConclusionsOur results provide reassurance about the influence of semi-automation as compared with the manual vitrification method. Concerning oocyte vitrification itself, no tight common transcriptomic signature associated has been observed across studies.Trial registrationNCT03570073.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

12888_2022_4451_Article_IEq2.gif

12888_2022_4365_Article_IEq48.gif

12982_2022_119_Article_IEq135.gif

12888_2022_4365_Article_IEq41.gif

12888_2022_4365_Article_IEq39.gif

12888_2022_4365_Article_IEq35.gif

12888_2022_4365_Article_IEq26.gif

12888_2022_4365_Article_IEq19.gif

12888_2022_4365_Article_IEq16.gif

12888_2022_4365_Article_IEq14.gif

Fig. 3

12982_2022_119_Article_IEq235.gif

12888_2022_4365_Article_IEq7.gif