Chromatin state distribution of residue-specific histone acetylation in early myoblast differentiation

Journal of Big Data

Brief Report

Yan Z. Mach¹  Yuan Li¹  Saadia Khilji¹  Qiao Li²  Jihong Chen³ 

[1] Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada;Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada;Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada;Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada;

关键词: Epigenetics;    Gene regulation;    Histone acetyltransferase;    Histone acetylation;    Stem cell differentiation;    Myogenesis;   

DOI  :  10.1186/s40537-022-00667-3

 received in 2022-02-10, accepted in 2022-11-29,  发布年份 2022

来源: Springer

【 摘 要 】

Dynamic changes in epigenetic landscape reflect a critical command of lineage-specific gene expression. In an effort to discern the epigenetic regulatory networks of myogenic differentiation, we have used systematic and integrative approaches to explore multi-omics datasets on global myogenic gene expression, histone acetylation and acetyltransferase occupancy in view of distinct chromatin states. In this brief report, we discuss experimental design and provide a comprehensive assessment regarding data quality control, filtering and processing. We also define a gene-level overlap between RNA-seq and ChIP-seq datasets through integrative analyses to offer strategies for future use of the data. Furthermore, our analyses generate a blueprint on chromatin state distribution of residue-specific histone acetylation and concomitant association with histone acetyltransferase p300 in committed skeletal myoblasts and differential histone acetylation signatures at the onset of myoblast differentiation. These datasets can be further utilized to delineate the function of muscle-specific regulatory elements governed by other muscle myogenic regulators or signaling molecules.

【 授权许可】

CC BY   
© The Author(s) 2022

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