Maternal dietary methionine restriction alters hepatic expression of one-carbon metabolism and epigenetic mechanism genes in the ducklings

BMC Genomics

Research

Aurélie Sécula¹  Laure Gress¹  Mireille Morisson¹  Agnès Bonnet¹  Hervé Chapuis¹  Lisa E. Bluy¹  Loys Bodin¹  Cécile M. D. Bonnefont¹  Anne Collin²  Xavier Martin³  Alexis Cornuez³ 

[1] GenPhySE, Université de Toulouse, INRAE, ENVT, F-31326, Castanet Tolosan, France;INRAE, Université de Tours, BOA, 37380, Nouzilly, France;UEPFG INRA Bordeaux-Aquitaine (Unité Expérimentale Palmipèdes à Foie Gras), Domaine d’Artiguères 1076, route de Haut Mauco, F-40280 Benquet, France;

关键词: Duck;    Methyl donor;    Nutritional programming;    Differentially expressed genes;    Avian;   

DOI  :  10.1186/s12864-022-09066-7

 received in 2022-07-12, accepted in 2022-12-05,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundEmbryonic and fetal development is very susceptible to the availability of nutrients that can interfere with the setting of epigenomes, thus modifying the main metabolic pathways and impacting the health and phenotypes of the future individual. We have previously reported that a 38% reduction of the methyl donor methionine in the diet of 30 female ducks reduced the body weight of their 180 mule ducklings compared to that of 190 ducklings from 30 control females. The maternal methionine-restricted diet also altered plasmatic parameters in 30 of their ducklings when compared to that of 30 ducklings from the control group. Thus, their plasma glucose and triglyceride concentrations were higher while their free fatty acid level and alanine transaminase activity were decreased. Moreover, the hepatic transcript level of 16 genes involved in pathways related to energy metabolism was significantly different between the two groups of ducklings. In the present work, we continued studying the liver of these newly hatched ducklings to explore the impact of the maternal dietary methionine restriction on the hepatic transcript level of 70 genes mostly involved in one-carbon metabolism and epigenetic mechanisms.ResultsAmong the 12 genes (SHMT1, GART, ATIC, FTCD, MSRA, CBS, CTH, AHCYL1, HSBP1, DNMT3, HDAC9 and EZH2) identified as differentially expressed between the two maternal diet groups (p-value < 0.05), 3 of them were involved in epigenetic mechanisms. Ten other studied genes (MTR, GLRX, MTHFR, AHCY, ADK, PRDM2, EEF1A1, ESR1, PLAGL1, and WNT11) tended to be differently expressed (0.05 < p-value < 0.10). Moreover, the maternal dietary methionine restriction altered the number and nature of correlations between expression levels of differential genes for one-carbon metabolism and epigenetic mechanisms, expression levels of differential genes for energy metabolism, and phenotypic traits of ducklings.ConclusionThis avian model showed that the maternal dietary methionine restriction impacted both the mRNA abundance of 22 genes involved in one-carbon metabolism or epigenetic mechanisms and the mRNA abundance of 16 genes involved in energy metabolism in the liver of the newly hatched offspring, in line with the previously observed changes in their phenotypic traits.

【 授权许可】

CC BY   
© The Author(s) 2022

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