Pinpointing genomic loci for drought-induced proline and hydrogen peroxide accumulation in bread wheat under field conditions

BMC Plant Biology

Research

Mekides Abebe Beyene¹  Agim Ballvora¹  Md Nurealam Siddiqui²  Ali Ahmad Naz³  Jens Léon⁴  Mohammad Kamruzzaman⁵ 

[1] Institute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of Bonn, Bonn, Germany;Institute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of Bonn, Bonn, Germany;Department of Biochemistry and Molecular Biology, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh;Institute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of Bonn, Bonn, Germany;Department of Plant Breeding, University of Applied Sciences, Osnabrueck, Osnabrueck, Germany;Institute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of Bonn, Bonn, Germany;Field Lab Campus Klein-Altendorf, University of Bonn, Bonn, Germany;Institute of Crop Science and Resource Conservation (INRES)-Plant Breeding and Biotechnology, University of Bonn, Bonn, Germany;Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh-2202, Bangladesh;

关键词: Drought;    Proline;    Hydrogen peroxide;    GWAS;    Wheat diversity;   

DOI  :  10.1186/s12870-022-03943-9

 received in 2022-08-13, accepted in 2022-11-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundProline (Pro) and hydrogen peroxide (H2O2) play a critical role in plants during drought adaptation. Genetic mapping for drought-induced Pro and H2O2 production under field conditions is very limited in crop plants since their phenotyping with large populations is labor-intensive. A genome-wide association study (GWAS) of a diversity panel comprised of 184 bread wheat cultivars grown in natural field (control) and rain-out shelter (drought) environments was performed to identify candidate loci and genes regulating Pro and H2O2 accumulation induced by drought.ResultsThe GWAS identified top significant marker-trait associations (MTAs) on 1A and 2A chromosomes, respectively for Pro and H2O2 in response to drought. Similarly, MTAs for stress tolerance index (STI) of Pro and H2O2 were identified on 5B and 1B chromosomes, respectively. Total 143 significant MTAs were identified including 36 and 71 were linked to drought and 2 and 34 were linked to STI for Pro and H2O2, respectively. Next, linkage disequilibrium analysis revealed minor alleles of significant single-markers and haplotypes were associated with higher Pro and H2O2 accumulation under drought. Several putative candidate genes for Pro and H2O2 content encode proteins with kinase, transporter or protein-binding activities.ConclusionsThe identified genetic factors associated with Pro and H2O2 biosynthesis underlying drought adaptation lay a fundamental basis for functional studies and future marker-assisted breeding programs.

【 授权许可】

CC BY   
© The Author(s) 2022

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