Overexpression of CENPL mRNA potentially regulated by miR-340-3p predicts the prognosis of pancreatic cancer patients

BMC Cancer

Research

Zhongzhuan Li¹  Hanfang Hu¹  Jiehong Xu¹  Gang Chen¹  Ling Du¹  Shiyu Ou¹  Zhongyuan Cui²  Zhixian Wu³  Jielong Wang⁴  Dongliang Li⁴  Shasha Li⁴ 

[1] Department of Gastroenterology, the Fourth Affiliated Hospital (Liuzhou Workers’ Hospital), Guangxi Medical University, 545000, Liuzhou, Guangxi, China;Department of Hepatobiliary Disease, 900th Hospital of the Joint Logistics Support Force (Dongfang Hospital), Xiamen University, 350025, Fuzhou, Fujian, China;Department of Hepatobiliary Disease, 900th Hospital of the Joint Logistics Support Force (Dongfang Hospital), Xiamen University, 350025, Fuzhou, Fujian, China;Department of Gastroenterology, the Fourth Affiliated Hospital (Liuzhou Workers’ Hospital), Guangxi Medical University, 545000, Liuzhou, Guangxi, China;Department of Hepatobiliary Disease, 900th Hospital of the Joint Logistics Support Force (Dongfang Hospital), Xiamen University, 350025, Fuzhou, Fujian, China;Department of Hepatobiliary Disease, 900th Hospital of the Joint Logistics Support Force, Fujian Medical University, 350025, Fuzhou, Fujian, China;

关键词: PAAD;    CENPL;    Biomarkers;    Prognosis;    miR-340-3p;   

DOI  :  10.1186/s12885-022-10450-5

 received in 2022-03-20, accepted in 2022-12-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIn our previous study it was found that CENPL was overexpressed in hepatocellular carcinoma and significantly predicted patient's prognosis. However, the expression and prognostic value of CENPL in other gastrointestinal tumors remain unknown. Therefore, we investigated the expression and prognostic value of CENPL in esophageal carcinoma (ESCA), stomach adenocarcinoma (STAD), pancreatic adenocarcinoma (PAAD), colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ).MethodsIn this study, Oncomine, GEPIA, OncoLnc, TIMER, cBioPortal, miRWalk and ENCORI databases were used to analyze the level of CENPL mRNA, prognostic value and potential regulatory mechanism of CENPL mRNA in tumors. The CENPL expression and clinicopathological data regarding PAAD were from the UCSC Xena database and univariate and multivariate Cox regression analyses were performed using R (Version 3.6.3). Immunohistochemical staining was used to verify the expression of CENPL protein in clinical specimens. Cytoscape (Version: 3.7.2) was used to visualize microRNA (miRNA) that potentially regulates CENPL.ResultsGene differential expression analysis showed that CENPL mRNA was significantly overexpressed in ESCA, STAD, PAAD, COAD and READ (p < 0.01). The overexpression of CENPL mRNA was significantly correlated with the poor prognosis of PAAD patients (p < 0.05). However, there was no significant correlation between the level of CENPL mRNA and the prognosis of ESCA, STAD, COAD and READ patients (p > 0.05). Univariate and multivariate Cox regression analyses suggested that CENPL was a prognostic risk factor for PAAD. The mutation rate of CENPL in PAAD was 2.2% (17/850). There was no significant correlation between the CENPL expression and the infiltration levels of immune cells in PAAD (|Cor|< 0.5). Immunohistochemical staining showed that CENPL was overexpressed in 42% (11/26) of PAAD specimens, which was significantly higher compared with that in the normal tissues. The expression of miR-340-3p and miR-484 in PAAD were significantly lower than in the normal tissues (p < 0.05) and PAAD patients with lower expression of miR-340-3p had poorer prognosis (p < 0.05).ConclusionCENPL potentially regulated by miR-340-3p, is overexpressed in PAAD and predicts patient’s prognosis, suggestive of a diagnostic and prognostic value in PAAD patients.

【 授权许可】

CC BY   
© The Author(s) 2022

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