Protocols for Plasmodium gametocyte production in vitro: an integrative review and analysis

Parasites & Vectors

Review

Michael Delves¹  Carlo Severini²  Roukayatou Omorou³  Yobouet Ines Kouakou³  Ibrahim Bin Sa’id⁴  Stephane Picot⁵  Anne-Lise Bienvenu⁶ 

[1] Department of Infection Biology, Faculty of Infectious Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, WC1A 7HT, London, UK;Dipartimento Di Malattie Infettive, Istituto Superiore Di Sanità, Rome, Italy;Malaria Research Unit, UMR 5246 CNRS-INSA-CPE-University Lyon1, University of Lyon, 69100, Villeurbanne, France;Malaria Research Unit, UMR 5246 CNRS-INSA-CPE-University Lyon1, University of Lyon, 69100, Villeurbanne, France;Institut Agama Islam Negeri (IAIN) Kediri, 64127, Kota Kediri, Jawa Timur, Indonesia;Malaria Research Unit, UMR 5246 CNRS-INSA-CPE-University Lyon1, University of Lyon, 69100, Villeurbanne, France;Institut de Parasitologie Et Mycologie Médicale, Groupement Hospitalier Nord, Hospices Civils de Lyon, Lyon, France;Malaria Research Unit, UMR 5246 CNRS-INSA-CPE-University Lyon1, University of Lyon, 69100, Villeurbanne, France;Service Pharmacie, Groupement Hospitalier Nord, Hospices Civils de Lyon, Lyon, France;

关键词: Plasmodium;    Malaria;    Gametocyte;    In vitro;    Ex vivo;    Protocols;    Transmission-blocking;   

DOI  :  10.1186/s13071-022-05566-3

 received in 2022-09-28, accepted in 2022-11-02,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundThe production of Plasmodium gametocytes in vitro is a real challenge. Many protocols have been described, but few have resulted in the production of viable and infectious gametocytes in sufficient quantities to conduct research on—but not limited to—transmission-blocking drug and vaccine development. The aim of this review was to identifyand discuss gametocyte production protocols that have been developed over the last two decades.MethodsWe analyzed the original gametocyte production protocols published from 2000 onwards based on a literature search and a thorough review. A systematic review was performed of relevant articles identified in the PubMed, Web of Sciences and ScienceDirect databases.ResultsA total 23 studies on the production of Plasmodium gametocytes were identified, 19 involving in vitro Plasmodium falciparum, one involving Plasmodium knowlesi and three involving ex vivo Plasmodium vivax. Of the in vitro studies, 90%used environmental stressors to trigger gametocytogenesis. Mature gametocytemia of up to 4% was reported.ConclusionsSeveral biological parameters contribute to an optimal production in vitro of viable and infectious mature gametocytes. The knowledge gained from this systematic review on the molecular mechanisms involved in gametocytogenesis enables reproducible gametocyte protocols with transgenic parasite lines to be set up. This review highlights the need for additional gametocyte production protocols for Plasmodium species other than P. falciparum.Graphical abstract

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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