Significance of NatB-mediated N-terminal acetylation of auxin biosynthetic enzymes in maintaining auxin homeostasis in Arabidopsis thaliana

Communications Biology

Article

Hai-Qing Liu¹  Li Zhang²  Allah Jurio Khaskheli³  Guang-Qin Guo³  Yu-Man Guo³  Jun-Li Wang³  Ya-Jie Zou³  Qiong-Hui Fei³  Peng Tian³  Xiao-Feng Li³  Lei Wu³  Zuo-Xian Pu³  Dong-Wei Di⁴ 

[1] MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China;MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China;Basic Forestry and Proteomics Research Center, College of Life Sciences, Fujian Agriculture and Forestry University, 350002, Fuzhou, China;MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China;Gansu Province Key Laboratory of Gene Editing for Breeding, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China;MOE Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, 730000, Lanzhou, China;Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China;

DOI  :  10.1038/s42003-022-04313-9

 received in 2019-11-29, accepted in 2022-11-29,  发布年份 2022

来源: Springer

【 摘 要 】

The auxin IAA (Indole-3-acetic acid) plays key roles in regulating plant growth and development, which depends on an intricate homeostasis that is determined by the balance between its biosynthesis, metabolism and transport. YUC flavin monooxygenases catalyze the rate-limiting step of auxin biosynthesis via IPyA (indole pyruvic acid) and are critical targets in regulating auxin homeostasis. Despite of numerous reports on the transcriptional regulation of YUC genes, little is known about those at the post-translational protein level. Here, we show that loss of function of CKRC3/TCU2, the auxiliary subunit (Naa25) of Arabidopsis NatB, and/or of its catalytic subunit (Naa20), NBC, led to auxin-deficiency in plants. Experimental evidences show that CKRC3/TCU2 can interact with NBC to form a NatB complex, catalyzing the N-terminal acetylation (NTA) of YUC proteins for their intracellular stability to maintain normal auxin homeostasis in plants. Hence, our findings provide significantly new insight into the link between protein NTA and auxin biosynthesis in plants.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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