Mouse model of subtalar post-traumatic osteoarthritis caused by subtalar joint instability

Journal of Orthopaedic Surgery and Research

Research Article

Kaiwen Chen¹  Jia Yu¹  Shuo Wang¹  Hongtao Zhang¹  Peixin Liu² 

[1] Department of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, 215006, Suzhou, Jiangsu, People’s Republic of China;Orthopaedic Institute, Medical College, Soochow University, 178 Ganjiangdong Rd, 215006, Suzhou, Jiangsu, People’s Republic of China;Department of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, 215006, Suzhou, Jiangsu, People’s Republic of China;Orthopaedic Institute, Medical College, Soochow University, 178 Ganjiangdong Rd, 215006, Suzhou, Jiangsu, People’s Republic of China;Department of Orthopedics, Suzhou Xiangcheng People’s Hospital, 1060 Huayuan Road, 215131, Suzhou, Jiangsu, People’s Republic of China;

关键词: Ankle sprain;    Ankle instability;    Subtalar instability;    Post-traumatic osteoarthritis;    Calcaneofibular ligament;   

DOI  :  10.1186/s13018-022-03435-4

 received in 2022-05-26, accepted in 2022-12-05,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundCommon ankle sprains are often accompanied by injury to the subtalar joint, which eventually leads to subtalar joint instability. Because the clinical manifestations for subtalar joint instability are similar to ankle joint injuries, these are often overlooked. This study aimed to establish an animal model of subtalar joint instability to study post-traumatic osteoarthritis of the subtalar joint caused by long-term subtalar joint instability and to provide a reference for future clinical research on chronic subtalar joint instability.MethodsIn all, 24 C57BL/6 male mice were randomly divided into three groups: Sham, cervical ligament (CL) transection and CL + calcaneofibular ligament (CFL) transection groups. One week after surgical operation, all mice were trained to run in the mouse rotation fatigue machine every day. During this period, a balance beam test was used to evaluate the motor level and coordination ability of the mice before the operation and three days, one week, four weeks, eight weeks, and twelve weeks after operation. Further, post-traumatic osteoarthritis of the subtalar joint was quantified via micro-CT and histological staining.ResultsThe mice in the partial ligament transection group took significantly longer than those in the Sham group to pass through the balance beam and showed an increased number of hindfoot slips. Micro-CT analysis showed that the subtalar bone volume fraction in the CL + CFL transection group and CL transection group was 5.8% and 2.8% higher than that in the Sham group, respectively. Histological staining showed obvious signs of post-traumatic osteoarthritis (PTOA) in the subtalar joint of the ligament transection group.ConclusionsThe transection of CL and CL + CFL can cause instability of the subtalar joint in mice, resulting in a decrease in motor coordination, and long-term instability of the subtalar joint in mice can cause PTOA of the subtalar joint, which is manifested as destruction and loss of articular cartilage.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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