A non-destructive approach for measuring rice panicle-level photosynthetic responses using 3D-image reconstruction

Plant Methods

Methodology

Balpreet Kaur Dhatt¹  Puneet Paul¹  Harkamal Walia¹  Jaspinder Singh Dharni¹  Paul Staswick¹  Tian Gao²  Hongfeng Yu²  Jason Hupp³  Tala Awada⁴  Zvi Peleg⁵  Harel Bacher⁵ 

[1] Department of Agronomy and Horticulture, University of Nebraska-Lincoln, 68583, Lincoln, NE, USA;Department of Computer Science and Engineering, University of Nebraska-Lincoln, 68588, Lincoln, NE, USA;LI-COR Inc., 4647 Superior Street, 68505, Lincoln, NE, USA;School of Natural Resources, University of Nebraska-Lincoln, 68583, Lincoln, NE, USA;The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel;

关键词: Rice;    Grain yield;    Panicle;    Carbon assimilation;    Imaging;    Heat stress;    Photosynthesis;    LiCOR 6800;   

DOI  :  10.1186/s13007-022-00959-y

 received in 2022-09-01, accepted in 2022-11-14,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundOur understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to the lack of a suitable gas exchange methodology for panicles and non-destructive methods to accurately determine panicle surface area.ResultsTo address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined using 3D panicle imaging to normalize the panicle-level photosynthetic measurements. We observed differential responses in both panicle and flag leaf for two temperate Japonica rice genotypes (accessions TEJ-1 and TEJ-2) exposed to heat stress during early grain filling. There was a notable divergence in the relative photosynthetic contribution of flag leaf and panicles for the heat-tolerant genotype (TEJ-2) compared to the sensitive genotype (TEJ-1).ConclusionThe novelty of this method is the non-destructive and accurate determination of panicle area and photosynthetic parameters, enabling researchers to monitor temporal changes in panicle physiology during the reproductive development. The method is useful for panicle-level measurements under diverse environmental stresses and is sensitive enough to evaluate genotypic variation for panicle physiology and architecture in cereals with compact inflorescences.

【 授权许可】

CC BY   
© The Author(s) 2022

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