The impact of Hyssop (Hyssopus officinalis) extract on activation of endosomal toll like receptors and their downstream signaling pathways

BMC Research Notes

Research Note

Amir Mehdizadeh¹  Leili Aghebati-Maleki²  Sanam Dolati³  Masoumeh Ghasempour⁴  Javad Ahmadian Heris⁴  Mehdi Yousefi⁵  Roza Motavalli⁶  Mohammad Sadegh Soltani-Zangbar⁷  Maryam Hosseini⁸ 

[1] Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Physical Medicine and Rehabilitation Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Tabriz, Iran;Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Allergy and Clinical Immunology, Pediatric Hospital, Tabriz University of Medical Sciences, Tabriz, Iran;Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;Trauma Research Center, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, Shiraz, Iran;

关键词: Hyssop;    TLR;    Immune system;    Proinflammatory cytokines;    IFN-I;   

DOI  :  10.1186/s13104-022-06253-3

 received in 2022-07-31, accepted in 2022-11-23,  发布年份 2022

来源: Springer

【 摘 要 】

ObjectivesFrom the ancient, medicinal benefits of Hyssop (Hyssopus officinalis L.) have been implicated for respiratory and digestive diseases despite the effects of Hyssop on viral infections have not been mechanistically investigated. In this study, we examined whether the Hyssop extract activated anti-viral innate immunity, as a sentinel for immune system, through activation of endosomal TLRs recognizing nucleic acids and their downstream signaling. The Hyssop herb extracts was prepared and co-cultured with healthy individual’s peripheral blood mononuclear cells (PBMCs). After viability assay, gene expression levels of TLR3,7,8,9, as well as MyD88 and NF-κB, were evaluated in treated PBMCs using Real-time PCR. Next, the secretion level of immune related cytokines was quantified via ELISA.ResultsPost 24 h, 40 µg/ml of the extract significantly inhibited the viability of less than 50% of cells compared to the control and had a maximum effect on cellular function. The Hyssop-treated PBMCs demonstrated an elevated expression of endosomal TLRs genes, as well as MyD88 and NF-κB. Moreover, the release of INF-α and β notably enhanced in cell culture supernatant, while the content of inflammatory cytokines remarkably diminished (P < 0.05). The Hyssop extract was capable of inducing antiviral innate immune responses so can be promising in antiviral drug strategies.

【 授权许可】

CC BY   
© The Author(s) 2022

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