microRNA-338-3p suppresses lipopolysaccharide-induced inflammatory response in HK-2 cells

BMC Molecular and Cell Biology

Research

Guokai Li¹  Jing Wang¹  Sheng Lin²  Ling Wu²  Min Lin³ 

[1] Department of nosocomial infection management, Fujian Maternity and Child Health Hospital, 350001, Fuzhou, Fujian, China;Department of pediatrics, Fujian Maternity and Child Health Hospital, No. 18 Daoshan Road, Gulou District, 350001, Fuzhou, Fujian, China;Pediatric intensive care unit, Fujian Maternity and Child Health Hospital, 350001, Fuzhou, Fujian, China;

关键词: MicroRNA;    Inflammation;    Kidney injury;    Lipopolysaccharide;    Mitochondrial membrane potential;   

DOI  :  10.1186/s12860-022-00455-0

 received in 2022-08-01, accepted in 2022-11-25,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundInflammation is the most common cause of kidney damage, and inflammatory responses in a number of diseases are mediated by microRNA-338-3p (miR-338-3p). However, there are only a few reports which described the regulation of miR-338-3p in human proximal tubular cells. The goal of this study was to see how miR-338-3p affected lipopolysaccharide (LPS)-caused inflammatory response in HK-2 cells.MethodsLPS was used to construct an inflammatory model in HK-2 cells. miR-338-3p mimic was used to increase the levels of miR-338-3p in HK-2 cells. MTT, JC-1 staining, and apoptosis assays were used to detect cell viability, mitochondrial membrane potential (MMP), and apoptosis, respectively. The production of inflammatory factors and the levels of p38, p65, phospho-p65, phospho-p38, Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were investigated using real-time polymerase chain reaction, western blotting, or enzyme-linked immunosorbent assay.ResultsThe levels of miR-338-3p were significantly lower in serum from patients with sepsis-induced kidney injury compared to the serum from healthy volunteers (P < 0.05). LPS reduced the level of miR-338-3p in HK-2 cells (P < 0.05). HK-2 cell viability, mitochondrial membrane potential, and Bcl-2 mRNA and protein levels were decreased by LPS (all P < 0.05). Apoptosis, the mRNA and protein levels of inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α) and Bax, and the levels of cleaved caspase-9 and caspase-3 were increased by LPS (all P < 0.05). Raising the level of miR-338-3p mitigated these effects of LPS (all P < 0.05).ConclusionLPS-induced inflammation in HK-2 cells is reduced by miR-338-3p.

【 授权许可】

CC BY   
© The Author(s) 2022

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