Transcriptome analysis and identification of chemosensory genes in the larvae of Plagiodera versicolora

BMC Genomics

Research

Jin-Meng Guo¹  Jian-Ting Fan²  Na Tong³  Xiao-Long Liu³  Min Lu³  Zhe-Ran Wu³  Yang Li³ 

[1] Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education/ Department of Entomology, Nanjing Agricultural University, 210095, Nanjing, China;School of Forestry and Biotechnology, Zhejiang A & F University, National Joint Local Engineering Laboratory for High-Efficient Preparation of Biopesticide, 311300, Lin’an, China;State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, 430062, Wuhan, China;

关键词: Plagiodera versicolora;    transcriptome;    odorant binding proteins;    chemosensory proteins;   

DOI  :  10.1186/s12864-022-09079-2

 received in 2022-07-26, accepted in 2022-12-12,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundIn insects, the chemosensory system is crucial in guiding their behaviors for survival. Plagiodera versicolora (Coleoptera: Chrysomelidae), is a worldwide leaf-eating forest pest in salicaceous trees. There is little known about the chemosensory genes in P. versicolora. Here, we conducted a transcriptome analysis of larvae heads in P. versicolora.ResultsIn this study, 29 odorant binding proteins (OBPs), 6 chemosensory proteins (CSPs), 14 odorant receptors (ORs), 13 gustatory receptors (GRs), 8 ionotropic receptors (IRs) and 4 sensory neuron membrane proteins (SNMPs) were identified by transcriptome analysis. Compared to the previous antennae and foreleg transcriptome data in adults, 12 OBPs, 2 CSPs, 5 ORs, 4 IRs, and 7 GRs were newly identified in the larvae. Phylogenetic analyses were conducted and found a new candidate CO2 receptor (PverGR18) and a new sugar receptor (PverGR23) in the tree of GRs. Subsequently, the dynamic expression profiles of various genes were analyzed by quantitative real-time PCR. The results showed that PverOBP31, OBP34, OBP35, OBP38, and OBP40 were highly expressed in larvae, PverOBP33 and OBP37 were highly expressed in pupae, and PverCSP13 was highly expressed in eggs, respectively.ConclusionsWe identified a total of 74 putative chemosensory genes based on a transcriptome analysis of larvae heads in P. versicolora. This work provides new information for functional studies on the chemoreception mechanism in P. versicolora.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

Fig. 1

Fig. 3

Fig. 5

Fig. 1

Fig. 2

Fig. 5