Pharmacokinetic study of the main components of Tanreqing capsules and Tanreqing injections in beagles by liquid chromatography–tandem mass spectrometry

Chinese Medicine

Research

Lili Cui¹  Fengjing Xu¹  Xia Tao¹  Deduo Xu¹  Shouhong Gao¹  Xinhua Song¹  Zhipeng Wang¹  Wansheng Chen¹  Yong Chen¹  Lifeng Huang²  Liang Wang³ 

[1] Department of Pharmacy, Second Affiliated Hospital of Naval Medical University, Shanghai, China;Department of Pharmacy, Suzhou Science & Technology Town Hospital, Suzhou, China;Suzhou Chien-Shiung Institute of Technology, Taicang, China;

关键词: Tanreqing capsule;    Tanreqing injection;    Pharmacokinetic parameters;    LC–MS/MS;    Beagles;   

DOI  :  10.1186/s13020-022-00690-9

 received in 2022-08-08, accepted in 2022-11-17,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundTanreqing capsules (TRQCs) and Tanreqing injections (TRQIs) are widely used in the treatment of respiratory diseases. In this study, a simple, rapid, and sensitive liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed for simultaneous quantification of the main components of Tanreqing, which include chlorogenic acid, ursodeoxycholic acid, chenodeoxycholic acid, and baicalin, in beagle dog plasma to compare their pharmacokinetic parameters.MethodsPlasma samples were pretreated with protein precipitation. Chromatographic separation was performed on Waters Acquity UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm) column using a gradient elution with (A) 0.1% (v/v) formic acid aqueous solution and (B) acetonitrile. Six healthy beagles were divided into two groups, and a crossover, comparative pharmacokinetic study of TRQC (0.09 g/kg) and TRQI (0.5 mL/kg) after a single-dose administration or daily doses over 7 days was carried out. One group was administrated a single dose of TRQC and followed continuously for 7 days, whereas the other group was treated with TRQI in the same way.ResultsThe calibration curves were linear over the ranges of 2.00–1000.00 ng/mL for baicalin, 10.00–5000.00 ng/mL for ursodeoxycholic acid, 1.00–500.00 ng/mLfor chenodeoxycholic acid and chlorogenic acid, respectively. The relative standard deviation of both intra-day and inter-day accuracy is less than 11.23%. The average extraction recovery of all compounds was greater than 82.21%. The major pharmacokinetic parameters of the four compounds were not significantly different between the two formulations (P > 0.05).ConclusionsThe measured levels of the four major components of TRQCs and TRQIs were comparable in these dogs, providing a reference for the clinical application of TRQCs instead of TRQIs.

【 授权许可】

CC BY   
© The Author(s) 2022

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