A decellularized and sterilized human meniscus allograft for off-the-shelf meniscus replacement

Journal of Experimental Orthopaedics

Original Paper

Manon E. Bertrand¹  Wietske Velthuijs²  Jasper Foolen³  Keita Ito³  Amal Mansoor³  Janne Spierings³  Jorge Alfredo Uquillas³  Rob P. A. Janssen⁴ 

[1] Hightech Contract Manufacturing Medical, Nijmegen, The Netherlands;Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600, Eindhoven, MB, the Netherlands;Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600, Eindhoven, MB, the Netherlands;Institute of Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, The Netherlands;Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, 5600, Eindhoven, MB, the Netherlands;Maxima Medical Centre Eindhoven/Veldhoven, Eindhoven, The Netherlands;Health Innovations and Technology, Department of Paramedical Sciences, Fontys University of Applied Sciences, Eindhoven, The Netherlands;

关键词: Meniscus;    Meniscal reconstruction;    Allograft;    Decellularization;    Supercritical carbon dioxide sterilization;   

DOI  :  10.1186/s40634-022-00555-y

 received in 2022-09-29, accepted in 2022-11-18,  发布年份 2022

来源: Springer

【 摘 要 】

PurposeMeniscus tears are one of the most frequent orthopedic knee injuries, which are currently often treated performing meniscectomy. Clinical concerns comprise progressive degeneration of the meniscus tissue, a change in knee biomechanics, and an early onset of osteoarthritis. To overcome these problems, meniscal transplant surgery can be performed. However, adequate meniscal replacements remain to be a great challenge. In this research, we propose the use of a decellularized and sterilized human meniscus allograft as meniscal replacement.MethodsHuman menisci were subjected to a decellularization protocol combined with sterilization using supercritical carbon dioxide (scCO2). The decellularization efficiency of human meniscus tissue was evaluated via DNA quantification and Hematoxylin & Eosin (H&E) and DAPI staining. The mechanical properties of native, decellularized, and decellularized + sterilized meniscus tissue were evaluated, and its composition was determined via collagen and glycosaminoglycan (GAG) quantification, and a collagen and GAG stain. Additionally, cytocompatibility was determined in vitro.ResultsHuman menisci were decellularized to DNA levels of ~ 20 ng/mg of tissue dry weight. The mechanical properties and composition of human meniscus were not significantly affected by decellularization and sterilization. Histologically, the decellularized and sterilized meniscus tissue had maintained its collagen and glycosaminoglycan structure and distribution. Besides, the processed tissues were not cytotoxic to seeded human dermal fibroblasts in vitro.ConclusionsHuman meniscus tissue was successfully decellularized, while maintaining biomechanical, structural, and compositional properties, without signs of in vitro cytotoxicity. The ease at which human meniscus tissue can be efficiently decellularized, while maintaining its native properties, paves the way towards clinical use.

【 授权许可】

CC BY   
© The Author(s) 2022

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