The impact of early-life antibiotics and probiotics on gut microbial ecology and infant health outcomes: a Pregnancy and Birth Cohort in Northwest China (PBCC) study protocol

BMC Pediatrics

Study Protocol

Wenfang Yang¹  Jing Zhou²  Amanuel Kidane Andegiorgish³  Zhonghai Zhu³  Xueyao Wang³  Lu Shi³  Liang Wang³  Qi Qi³  Jiali Shen³  Yingze Zhu³  Mitslal Abrha Gebremedhin³  Yijun Kang³  Yue Cheng³  Shaoru Li³  Lingxia Zeng⁴  Ying Yang⁵ 

[1] Department of Obstetrics and Gynaecology, The First Affiliated Hospital of Xi’an Jiaotong University, Maternal & Child Health Centre, Xi’an, Shaanxi, People’s Republic of China;Department of Paediatrics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China;School of Public Health, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, People’s Republic of China;School of Public Health, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, People’s Republic of China;Key Laboratory of Environment and Genes Related to Diseases (Xi’an Jiaotong University), Ministry of Education, Xi’an, Shaanxi, People’s Republic of China;Xi’an Lintong District Maternal and Child Health Hospital, Xi’an, Shaanxi, People’s Republic of China;

关键词: Cohort;    Antibiotics;    Probiotics;    Microbiota;    Maternal and child health;   

DOI  :  10.1186/s12887-022-03811-3

 received in 2021-12-22, accepted in 2022-12-18,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundUnreasonable use of antibiotics and probiotics can alter the gut ecology, leading to antibiotic resistance and suboptimal health outcomes during early life. Our study aims are to clarify the association among antibiotic and probiotic exposure in early life, the microecology of the gut microbiota, and the development of antibiotic resistance; to investigate the long-term impact of antibiotics and probiotics on the health outcomes of infants and young children; and to provide a theoretical basis for the rational use of antibiotics and probiotics from a life course perspective.MethodsThe study is a prospective, longitudinal birth cohort study conducted in Shaanxi Province, China from 2018 to 2024. A total of 3,000 eligible mother–child pairs will be enrolled from rural, suburban, and urban areas. The recruitment of the participants begins at pregnancy, and the newborns will be followed up for 2 years at successive timepoints: within 3 days after birth, 42 days after birth, and at 3, 6, 12, 18, and 24 months of age. Sociodemographic data, environmental exposures, dietary patterns, psychological conditions, and medical and drug histories are collected. Cognitive and behavioural development among infants and young children and questionnaires on antibiotic knowledge and behaviour among caregivers will be collected at 12 and 24 months of age. The faecal samples are collected and analysed by 16S rRNA high-throughput sequencing and quantitative PCR (qPCR) for antibiotic resistance genes.DiscussionThe findings will inform antibiotic and probiotic use for pregnant women and infants and contribute to establishing rational use strategies of antibiotics and probiotics for paediatricians, health practitioners, and drug administration policy-makers.Trial registrationThe study was registered on the Chinese Clinical Trial Registry (ChiCTR) platform, http://www.chictr.org.cn (Record ID: ChiCTR2100047531, June 20, 2021).

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

Fig. 1

12982_2022_119_Article_IEq160.gif

12982_2022_119_Article_IEq156.gif