Formulation enhanced the stability of Foot-and-mouth virus and prolonged vaccine storage

Virology Journal

Research

Yanyan Chang¹  Yanming Wei²  Jing Li³  Shunli Yang⁴  Guangqing Zhou⁴ 

[1] China Agricultural Vet.Bio.Science and Technology Co, Ltd, Lanzhou, China;College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China;College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China;China Agricultural Vet.Bio.Science and Technology Co, Ltd, Lanzhou, China;State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences, Lanzhou, People’s Republic of China;

关键词: FMDV;    Formulation;    Stability;    Vaccine;    Shelf life;   

DOI  :  10.1186/s12985-022-01928-6

 received in 2022-05-10, accepted in 2022-11-14,  发布年份 2022

来源: Springer

【 摘 要 】

Foot-and-mouth disease (FMD) is a highly contagious viral disease that affects cloven-hoofed animals. Vaccination is the most effective measure to control FMD. However, FMDV particles are prone to dissociation, leading to insufficient potency of vaccine. Based on this characteristic, a combination of twenty percentage trehalose, 500 mM NaCl and 3 mM CuSO4·5H2O was developed to increase viral stability. Heating-resistance test showed that FMDV infectivity was maintained when formulated with formulation. Additionally, the half-life of FMDV inactivation was prolonged remarkably. Sequencing analysis demonstrated that viral genome could not be altered in serial passages. Vaccine stability was monitored for up to 1 year at 4 °C, with a higher level of 146S content remained. This study suggested that the formulation could protect FMDV against massive structural breakdown and extend the shelf life of vaccine. Our findings could provide strategy to develop more solutions for the stabilization of viral vaccine.

【 授权许可】

CC BY   
© The Author(s) 2022

【 图 表 】

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